Brown J L, Mucci D, Whiteley M, Dirksen M L, Kassis J A
Laboratory of Developmental Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA.
Mol Cell. 1998 Jun;1(7):1057-64. doi: 10.1016/s1097-2765(00)80106-9.
Genes of the Polycomb group (PcG) of Drosophila encode proteins necessary for the maintenance of transcriptional repression of homeotic genes. PcG proteins are thought to act by binding as multiprotein complexes to DNA through Polycomb group response elements (PREs); however, specific DNA binding has not been demonstrated for any of the PcG proteins. We have identified a sequence-specific DNA binding protein that interacts with a PRE from the Drosophila engrailed gene. This protein (PHO) is a homolog of the ubiquitous mammalian transcription factor Yin Yang-1 and is encoded by pleiohomeotic, a known member of the PcG. We propose that PHO acts to anchor PcG protein complexes to DNA.
果蝇的多梳蛋白家族（PcG）基因编码维持同源异型基因转录抑制所必需的蛋白质。PcG蛋白被认为是通过多蛋白复合物经多梳蛋白家族反应元件（PREs）与DNA结合来发挥作用的；然而，尚未证实任何一种PcG蛋白具有特异性DNA结合能力。我们鉴定出一种序列特异性DNA结合蛋白，它与果蝇engrailed基因的一个PRE相互作用。这种蛋白（PHO）是普遍存在的哺乳动物转录因子阴阳1的同源物，由多梳蛋白家族的已知成员多效同源基因编码。我们提出，PHO的作用是将PcG蛋白复合物锚定到DNA上。
