Fellenberag A J, Pollard A C
Clin Chim Acta. 1976 Jun 15;69(3):423-8. doi: 10.1016/0009-8981(76)90114-5.
An ultraviolet spectrophotometric procedure for the micro determination of carbamazepine in blood is described which is based on the original 9-methyl-acridine method proposed by Beyer, K.H. and Klinge, K. (1969) (Arzneim-Forsch. 19, 1759--1760). Carbamazepine is extracted from blood with dichloromethane, which is then washed with alkali and acid. An aliquot of the extractant is evaporated to dryness and the residue heated briefly with hydrochloric acid at 150 degrees C. Following removal of non-specific interference with n-heptane, the absorbance of the acid catalysed rearrangement product (9-methylacridine) is determined at 258 nm. The resulting procedure is rapid, reliable, sensitive and specific. It requires 100-200 mul sample for a single estimation and has a detection threshold of less than 0.1 mg/100 ml. It is concluded that the method is suitable for routine clinical use.
本文描述了一种用于微量测定血液中卡马西平的紫外分光光度法，该方法基于Beyer, K.H.和Klinge, K.（1969年）提出的原始9-甲基吖啶法（《药物研究》19, 1759 - 1760）。用二氯甲烷从血液中提取卡马西平，然后用碱和酸洗涤。取一部分萃取剂蒸发至干，残余物在150℃下用盐酸短暂加热。用正庚烷去除非特异性干扰后，在258nm处测定酸催化重排产物（9-甲基吖啶）的吸光度。所得方法快速、可靠、灵敏且特异。单次测定需要100 - 200微升样品，检测限低于0.1mg/100ml。结论是该方法适用于常规临床应用。
