Fellenberg A J, Pollard A C
Clin Chim Acta. 1979 Mar 1;92(2):267-72. doi: 10.1016/0009-8981(79)90122-0.
An ultraviolet spectrophotometric procedure for the microdetermination of theophylline in plasma is described. Theophylline is extracted at pH 5 with chloroform. An aliquot of the solvent is shaken with molar hydrochloric acid and the absorbance of the acid layer determined at 267 nm. The resulting procedure is rapid, reliable, sensitive and virtually specific. It requires 250 microliters sample for a single estimation and has a detection threshold of less than 5 mumol/l.
本文描述了一种用于微量测定血浆中茶碱的紫外分光光度法。在pH 5条件下用氯仿萃取茶碱。取一份溶剂等分试样与摩尔盐酸振荡，然后测定酸层在267 nm处的吸光度。所得方法快速、可靠、灵敏且几乎具有特异性。单次测定需要250微升样品，检测限小于5微摩尔/升。
