Sato S, Tomita I
Toxicology Research Laboratories, Japan Tobacco Inc., Hatano-shi.
Biol Pharm Bull. 1998 Jan;21(1):90-2. doi: 10.1248/bpb.21.90.
Strain differences of mice in the induction of DNA damage in peripheral blood cells and skin tumors were investigated using 7,12-dimethylbenz[a]anthracene (DMBA). DMBA-induced DNA damage and skin tumorigenesis were evaluated using the single cell gel electrophoresis (SCGE) assay and 2-stage carcinogenicity study, respectively. DNA damaged cells were markedly increased in the aryl hydrocarbon hydroxylase (AHH)-inducible mice, BALB/c and C57BL/6, as compared with the AHH-noninducible mice, DBA/2, in the SCGE assay. The AHH-inducible mice were more sensitive to DMBA than the AHH-noninducible mice in the 2-stage carcinogenicity study. These results strongly suggest that the genetic capacity to metabolize PAH is associated with the mutagenicity and carcinogenicity of DMBA.
使用7,12-二甲基苯并[a]蒽（DMBA）研究了小鼠在外周血细胞DNA损伤诱导和皮肤肿瘤发生方面的品系差异。分别使用单细胞凝胶电泳（SCGE）分析和两阶段致癌性研究评估DMBA诱导的DNA损伤和皮肤肿瘤发生。在SCGE分析中，与芳烃羟化酶（AHH）不可诱导的小鼠DBA/2相比，AHH可诱导的小鼠BALB/c和C57BL/6中DNA受损细胞明显增加。在两阶段致癌性研究中，AHH可诱导的小鼠对DMBA比AHH不可诱导的小鼠更敏感。这些结果有力地表明，代谢多环芳烃的遗传能力与DMBA的诱变性和致癌性有关。
