Ogura K, Fukuzawa S, Habuchi T, Ogawa O, Yoshida O
Department of Urology, Rakuwa-kai Otowa Hospital, Faculty of Medicine, Kyoto University, Japan.
Int J Urol. 1997 Nov;4(6):561-6. doi: 10.1111/j.1442-2042.1997.tb00309.x.
In an attempt to determine the biological significance of nuclear morphometric findings, measurements of mean nuclear volume (MNV) and nuclear roundness factor (NRF) were compared to the immunoreactivity of p53 expression and proliferating cell nuclear antigen (PCNA) in human bladder cancer.
MNV and NRF were measured using stereological methods. Expression of p53 and PCNA were determined by immunohistochemical staining. Specimens from 111 patients with previously untreated bladder cancer were analyzed.
The mean MNV was 235.8 +/- 133.6 microm3 for the 81 patients with p53-labeling index (LI) less than 10% and 337.2 +/- 141.0 microm3 for the 30 patients with p53 LI greater than 10% (P = 0.008). There was no significant correlation between NRF and expression of p53. The mean MNV was 220.1 +/- 120.5 microm3 for the 67 patients with PCNA LI less than 28% (the mean value of PCNA LI) and 328.9 +/- 149.2 microm3 in 44 patients with PCNA LI greater than 28% (P = 0.0001). The mean NRF was 80.7 +/- 4.2 for the 67 patients with PCNA LI less than 28%, and 82.3 +/- 3.4 for the 44 patients with PCNA LI more than 28% (P = 0.04).
Nuclear morphometric findings may reflect the proliferative potential of cancer cells of the bladder, as indicated by findings of immunostaining for p53 and PCNA.
为了确定细胞核形态测量结果的生物学意义，在人类膀胱癌中，将平均核体积（MNV）和核圆度因子（NRF）的测量结果与p53表达和增殖细胞核抗原（PCNA）的免疫反应性进行了比较。
采用体视学方法测量MNV和NRF。通过免疫组织化学染色确定p53和PCNA的表达。分析了111例未经治疗的膀胱癌患者的标本。
81例p53标记指数（LI）小于10%的患者的平均MNV为235.8±133.6立方微米，30例p53 LI大于10%的患者的平均MNV为337.2±141.0立方微米（P = 0.008）。NRF与p53表达之间无显著相关性。67例PCNA LI小于28%（PCNA LI的平均值）的患者的平均MNV为220.1±120.5立方微米，44例PCNA LI大于28%的患者的平均MNV为328.9±149.2立方微米（P = 0.0001）。67例PCNA LI小于28%的患者的平均NRF为80.7±4.2，44例PCNA LI大于28%的患者的平均NRF为82.3±3.4（P = 0.04）。
正如p53和PCNA免疫染色结果所示，细胞核形态测量结果可能反映膀胱癌细胞的增殖潜能。
