Griffin M J
In Vitro. 1976 May;12(5):393-8. doi: 10.1007/BF02796317.
A technique was investigated for producing parasynchronous growth of some established, aneuploid human cell strains. Removal of both serum and calcium from exponentially growing monolayer cells tended to inhibit their growth. After 20 hr, a high percentage of the cell population was arrested in or near mitosis. Readdition of serum and calcium caused parasynchronous growth of the cells of three human strains studied. All three strains incorporated tritiated thymidine maximally 10 to 15 hr after serum and calcium were added, and cell numbers increased rapidly 17 to 25 hr after the growth medium was reconstituted. Population-doubling ranged from 80% to 100% of the theoretical. The yield of parasynchronous cells is high with this technique and may produce a significant amount of nontemporally distorted biological material upon which direct biochemical analysis can be performed at various times within the generation cycle.
研究了一种促使某些已建立的非整倍体人类细胞株同步生长的技术。从指数生长的单层细胞中去除血清和钙往往会抑制其生长。20小时后，高比例的细胞群体停滞在有丝分裂期或接近有丝分裂期。重新添加血清和钙可使所研究的三个人类细胞株的细胞同步生长。在添加血清和钙后，所有三个细胞株在10至15小时时最大程度地掺入了氚标记的胸腺嘧啶核苷，并且在重新配制生长培养基后17至25小时细胞数量迅速增加。群体倍增幅度为理论值的80%至100%。用这种技术获得的同步细胞产量很高，并且可能产生大量未受时间扭曲的生物材料，可在代周期内的不同时间对其进行直接生化分析。
