Thomas D, Kim H Y, Morgan R, Hanley M R
Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA.
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):599-603. doi: 10.1042/bj3300599.
Expression of the double-stranded-RNA-dependent protein kinase (PKR) in Xenopus oocytes attenuated Ca2+ entry-dependent membrane currents activated by depletion of Ca2+ stores, whereas expression of a dominant-negative PKR mutant had the opposite effect. These results appeared to be due to perturbation of releasable Ca2+ stores, and not actions of PKR on protein synthesis. PKR may thus have novel protein substrates and cellular functions in Ca2+ storage and signalling.
双链RNA依赖性蛋白激酶（PKR）在非洲爪蟾卵母细胞中的表达减弱了因钙库耗竭而激活的钙内流依赖性膜电流，而显性负性PKR突变体的表达则产生相反的效果。这些结果似乎是由于可释放钙库受到干扰，而非PKR对蛋白质合成的作用。因此，PKR在钙储存和信号传导方面可能具有新的蛋白质底物和细胞功能。
