在卵母细胞成熟过程中 G 蛋白雌激素受体（GPER）在细胞膜上的表达。
Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation.
机构信息
Department of Obstetrics and Gynecology, Ningxia Medical University, Yinchuan, China.
出版信息
J Assist Reprod Genet. 2013 Feb;30(2):227-32. doi: 10.1007/s10815-013-9942-z.
Abstract
PURPOSE
To determine expression of G-protein estrogen receptor (GPER) in mouse oocyte membrane during maturation.
METHODS
The expression of GPER from different maturation stages of oocytes, in vivo and in vitro matured oocytes as well as aging oocytes was examined by immune-fluorescence GPR30 antibody and the images were analyzed by laser scanning confocal microscope. Further confirmation was performed by Western blots for cell fractionation.
RESULTS
Significant fluorescent signal was observed on the surface of mouse oocytes. The image expression was lower in germinal vesicle (GV) stage than mature metaphase-II (M-II) stage oocytes. There was high expression in in-vivo matured oocytes compared to in vitro matured oocytes. The highest expression was observed in aging oocytes compared with other oocytes.
CONCLUSIONS
The changes of expression of GPER on mouse oocytes plasma membrane confirm oocyte membrane maturation, suggesting that those changes of GPER may be related to the functional role of oocyte maturation.
摘要
目的
确定 G 蛋白雌激素受体（GPER）在小鼠卵母细胞膜中的成熟过程中的表达。
方法
通过免疫荧光 GPR30 抗体检测不同成熟阶段卵母细胞、体内和体外成熟卵母细胞以及老化卵母细胞中 GPER 的表达，并通过激光共聚焦扫描显微镜分析图像。通过细胞分级分离的 Western blot 进行进一步确认。
结果
在小鼠卵母细胞表面观察到明显的荧光信号。与成熟中期（M-II）阶段卵母细胞相比，在生发泡（GV）期卵母细胞中的图像表达较低。与体外成熟卵母细胞相比，体内成熟卵母细胞中的表达较高。与其他卵母细胞相比，老化卵母细胞中的表达最高。
结论
小鼠卵母细胞膜上 GPER 表达的变化证实了卵母细胞膜的成熟，表明 GPER 的这些变化可能与卵母细胞成熟的功能作用有关。
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