编码两种人类α类谷胱甘肽转移酶（GSTA3和GSTA4）的cDNA的鉴定以及GSTA4-4的异源表达。
Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4.
作者信息
Board P G
机构信息
John Curtin School of Medical Research, Australian National University, GPO Box 334, Canberra ACT 2601, Australia.
出版信息
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):827-31. doi: 10.1042/bj3300827.
Abstract
The Expressed Sequence Tag database has been searched for examples of previously undescribed human Alpha class glutathione transferases. An incomplete transcript of the previously described GSTA3 gene was identified in a cDNA library derived from 8-9 week placenta. This indicates that the GSTA3 gene is functional and is possibly under specific developmental regulation. A second cDNA, termed GSTA4, was identified in a brain cDNA library. The encoded GSTA4-4 enzyme was expressed in Escherichia coli and was found to be immunologically distinct from GSTA1-1 and to have high activity with alk-2-enals. Although GSTA4-4 appears to be functionally similar to the mouse GST5.7 and rat GST8-8 Alpha class enzymes, sequence comparisons and phylogenetic analysis suggest that GSTA4-4 may be a member of a distinct Alpha class subgroup.
摘要
已在表达序列标签数据库中搜索先前未描述的人类α类谷胱甘肽转移酶的实例。在一个源自8 - 9周胎盘的cDNA文库中鉴定出了先前描述的GSTA3基因的一个不完整转录本。这表明GSTA3基因具有功能，并且可能受到特定的发育调控。在一个脑cDNA文库中鉴定出了第二个cDNA，称为GSTA4。编码的GSTA4 - 4酶在大肠杆菌中表达，发现其在免疫上与GSTA1 - 1不同，并且对alk - 2 -烯醛具有高活性。尽管GSTA4 - 4在功能上似乎与小鼠GST5.7和大鼠GST8 - 8α类酶相似，但序列比较和系统发育分析表明，GSTA4 - 4可能是一个独特的α类亚组的成员。
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