Board P G
John Curtin School of Medical Research, Australian National University, GPO Box 334, Canberra ACT 2601, Australia.
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):827-31. doi: 10.1042/bj3300827.
The Expressed Sequence Tag database has been searched for examples of previously undescribed human Alpha class glutathione transferases. An incomplete transcript of the previously described GSTA3 gene was identified in a cDNA library derived from 8-9 week placenta. This indicates that the GSTA3 gene is functional and is possibly under specific developmental regulation. A second cDNA, termed GSTA4, was identified in a brain cDNA library. The encoded GSTA4-4 enzyme was expressed in Escherichia coli and was found to be immunologically distinct from GSTA1-1 and to have high activity with alk-2-enals. Although GSTA4-4 appears to be functionally similar to the mouse GST5.7 and rat GST8-8 Alpha class enzymes, sequence comparisons and phylogenetic analysis suggest that GSTA4-4 may be a member of a distinct Alpha class subgroup.
已在表达序列标签数据库中搜索先前未描述的人类α类谷胱甘肽转移酶的实例。在一个源自8 - 9周胎盘的cDNA文库中鉴定出了先前描述的GSTA3基因的一个不完整转录本。这表明GSTA3基因具有功能，并且可能受到特定的发育调控。在一个脑cDNA文库中鉴定出了第二个cDNA，称为GSTA4。编码的GSTA4 - 4酶在大肠杆菌中表达，发现其在免疫上与GSTA1 - 1不同，并且对alk - 2 -烯醛具有高活性。尽管GSTA4 - 4在功能上似乎与小鼠GST5.7和大鼠GST8 - 8α类酶相似，但序列比较和系统发育分析表明，GSTA4 - 4可能是一个独特的α类亚组的成员。
