由黄素腺嘌呤二核苷酸（FAD）通过酶促反应生成核黄素4',5'-环磷酸酯：大鼠肝脏中一种特定的低Km黄素单核苷酸（FMN）环化酶的证据1。
Enzymic formation of riboflavin 4',5'-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1.
作者信息
Fraiz F J, Pinto R M, Costas M J, Aavalos M, Canales J, Cabezas A, Cameselle J C
机构信息
Unidad de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Extremadura, Apartado 108, E-06080 Badajoz, Spain.
出版信息
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):881-8. doi: 10.1042/bj3300881.
Abstract
An enzyme activity splitting FAD to AMP and riboflavin 4',5'-cyclic phosphate (4',5'-cFMN), with a Km of 6-8 microM, was partially purified from the cytosolic fraction of rat liver homogenates. 4', 5'-cFMN was characterized by enzyme, HPLC, UV-visible and NMR spectroscopic analyses. The data suggest that a novel enzyme, tentatively named FAD-AMP lyase (cyclizing) or FMN cyclase, is involved. Also, 4',5'-cFMN was hydrolysed to 5'-FMN by a rat liver cyclic phosphodiesterase. The results indicate a novel enzymic pathway for flavins in mammals, and support the biological relevance of 4',5'-cFMN, perhaps as a flavocoenzyme or a regulatory signal.
摘要
从大鼠肝脏匀浆的胞质部分中部分纯化出一种酶活性，该酶可将黄素腺嘌呤二核苷酸（FAD）分解为腺苷酸（AMP）和核黄素4',5'-环磷酸酯（4',5'-cFMN），其米氏常数（Km）为6 - 8微摩尔。通过酶分析、高效液相色谱（HPLC）、紫外可见光谱和核磁共振（NMR）光谱分析对4',5'-cFMN进行了表征。数据表明，涉及一种新酶，暂命名为FAD - AMP裂解酶（环化）或FMN环化酶。此外，大鼠肝脏环磷酸二酯酶可将4',5'-cFMN水解为5'-FMN。结果表明哺乳动物体内黄素存在一种新的酶促途径，并支持4',5'-cFMN的生物学相关性，其可能作为黄素辅酶或调节信号。
相似文献
1
Enzymic formation of riboflavin 4',5'-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1.由黄素腺嘌呤二核苷酸（FAD）通过酶促反应生成核黄素4',5'-环磷酸酯：大鼠肝脏中一种特定的低Km黄素单核苷酸（FMN）环化酶的证据1。
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):881-8. doi: 10.1042/bj3300881.
2
Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phosphodiester of X, either a monocyclic compound or a cis-bicyclic phosphodiester-pyranose fusion.大鼠肝脏FAD-AMP裂解酶（环化）的纯化、特性鉴定以及底物和抑制剂的构效关系研究：对FAD的偏好性以及将核糖核苷二磷酸-X裂解为核糖核苷酸和X的五元环磷酸二酯（单环化合物或顺式双环磷酸二酯-吡喃糖融合物）的特异性。
Biochemistry. 2001 Nov 13;40(45):13710-22. doi: 10.1021/bi0157159.
3
Preparation of riboflavin 4',5'-cyclic phosphate by incubation of flavin-adenine dinucleotide with Mn2+ in the absence of riboflavin 5'-phosphate cyclase.
Anal Biochem. 1999 Mar 15;268(2):409-11. doi: 10.1006/abio.1998.3063.
4
Fluorimetric HPLC detection of endogenous riboflavin 4',5'-cyclic phosphate in rat liver at nanomolar concentrations.
Anal Biochem. 2005 Jun 15;341(2):214-9. doi: 10.1016/j.ab.2005.01.060.
5
Flavin adenine dinucleotide and flavin mononucleotide metabolism in rat liver--the occurrence of FAD pyrophosphatase and FMN phosphohydrolase in isolated mitochondria.大鼠肝脏中黄素腺嘌呤二核苷酸和黄素单核苷酸代谢——分离线粒体中FAD焦磷酸酶和FMN磷酸水解酶的存在情况
Eur J Biochem. 1997 Nov 1;249(3):777-85. doi: 10.1111/j.1432-1033.1997.00777.x.
6
Evidence for the presence of a FAD pyrophosphatase and a FMN phosphohydrolase in yeast mitochondria: a possible role in flavin homeostasis.酵母线粒体中 FAD 焦磷酸酶和 FMN 磷酸水解酶的存在证据：在黄素稳态中的可能作用。
Yeast. 2011 Oct;28(10):693-705. doi: 10.1002/yea.1897. Epub 2011 Sep 13.
7
Flavin levels in the rat retina.大鼠视网膜中的黄素水平。
Exp Eye Res. 1992 Apr;54(4):605-9. doi: 10.1016/0014-4835(92)90139-j.
8
A bifunctional molecule as an artificial flavin mononucleotide cyclase and a chemosensor for selective fluorescent detection of flavins.一种双功能分子，作为人工黄素单核苷酸环化酶和用于黄素选择性荧光检测的化学传感器。
J Am Chem Soc. 2009 Jul 29;131(29):10107-12. doi: 10.1021/ja9018012.
9
Metabolism of injected flavins studied by using double-labeled [14C]flavin adenine dinucleotide and [14C, 32P]flavin mononucleotide.通过使用双标记的[14C]黄素腺嘌呤二核苷酸和[14C, 32P]黄素单核苷酸研究注射黄素的代谢。
J Nutr Sci Vitaminol (Tokyo). 1978;24(5):505-10. doi: 10.3177/jnsv.24.505.
10
Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases.鉴定人源和大鼠源FAD-AMP裂解酶（形成环状FMN）为ATP依赖性二羟基丙酮激酶。
Biochem Biophys Res Commun. 2005 Dec 30;338(4):1682-9. doi: 10.1016/j.bbrc.2005.10.142. Epub 2005 Nov 2.
引用本文的文献
1
Alternative Splicing of the Last Intron Yields Transcripts Differentially Expressed in Human Tissues That Code In Vitro for a Protein Devoid of Triokinase and FMN Cyclase Activity.最后一个内含子的可变剪接产生在人体组织中差异表达的转录本，这些转录本编码的体外蛋白缺乏三激酶和 FMN 环化酶活性。
Biomolecules. 2024 Oct 12;14(10):1288. doi: 10.3390/biom14101288.
2
Proteomic changes associated with metabolic syndrome in a fructose-fed rat model.与果糖喂养大鼠模型代谢综合征相关的蛋白质组学变化。
J Food Drug Anal. 2016 Oct;24(4):754-761. doi: 10.1016/j.jfda.2016.03.005. Epub 2016 Jun 24.
3
Does Abiotic Stress Cause Functional B Vitamin Deficiency in Plants?非生物胁迫会导致植物功能性B族维生素缺乏吗？
Plant Physiol. 2016 Dec;172(4):2082-2097. doi: 10.1104/pp.16.01371. Epub 2016 Nov 2.
4
Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements.双功能同源二聚体三激酶/FMN 环化酶：蛋白质结构域对人源酶活性的贡献及结构域运动的分子动力学模拟。
J Biol Chem. 2014 Apr 11;289(15):10620-10636. doi: 10.1074/jbc.M113.525626. Epub 2014 Feb 25.
本文引用的文献
1
Adenylyl cyclases: structure, regulation and function in an enzyme superfamily.
Mol Cell Endocrinol. 1997 Apr 4;128(1-2):179-94. doi: 10.1016/s0303-7207(97)04013-6.
2
Biogenesis of the covalently flavinylated mitochondrial enzyme dimethylglycine dehydrogenase.
J Biol Chem. 1996 Apr 19;271(16):9823-9. doi: 10.1074/jbc.271.16.9823.
3
Synthesis and degradation of cyclic ADP-ribose by NAD glycohydrolases.NAD 糖水解酶对环状 ADP - 核糖的合成与降解
Science. 1993 Sep 3;261(5126):1330-3. doi: 10.1126/science.8395705.
4
Cyclic ADP-ribose: a new way to control calcium.环磷酸腺苷核糖：一种控制钙的新途径。
Science. 1993 Jan 15;259(5093):325-6. doi: 10.1126/science.8380506.
5
Biosynthesis and function of enzymes with covalently bound flavin.具有共价结合黄素的酶的生物合成与功能
Annu Rev Nutr. 1993;13:17-41. doi: 10.1146/annurev.nu.13.070193.000313.
6
Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38.淋巴细胞抗原CD38催化的环ADP核糖的形成与水解
Science. 1993 Nov 12;262(5136):1056-9. doi: 10.1126/science.8235624.
7
Cyclic ADP-ribose: a new member of a super family of signalling cyclic nucleotides.环磷酸腺苷核糖：信号性环核苷酸超家族的新成员。
Cell Signal. 1994 Aug;6(6):591-600. doi: 10.1016/0898-6568(94)90042-6.
8
Purification and characterization of flavine-adenine dinucleotide phosphohydrolase from rat liver lysosomal membranes.大鼠肝脏溶酶体膜中黄素腺嘌呤二核苷酸磷酸水解酶的纯化与特性分析
J Biochem. 1993 Jul;114(1):126-31. doi: 10.1093/oxfordjournals.jbchem.a124127.
9
Flavinylation of monoamine oxidase B.单胺氧化酶B的黄素化
J Biol Chem. 1995 Oct 6;270(40):23653-60. doi: 10.1074/jbc.270.40.23653.
10
Structural and dynamic information on the complex of Megasphaera elsdenii apoflavodoxin and riboflavin 5'-phosphate. A phosphorus-31 nuclear magnetic resonance study.
Biochemistry. 1982 Jan 19;21(2):408-14. doi: 10.1021/bi00531a031.
Zotero 插件