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2
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6
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7
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由黄素腺嘌呤二核苷酸（FAD）通过酶促反应生成核黄素4',5'-环磷酸酯：大鼠肝脏中一种特定的低Km黄素单核苷酸（FMN）环化酶的证据1。

Enzymic formation of riboflavin 4',5'-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1.

作者信息

Fraiz F J, Pinto R M, Costas M J, Aavalos M, Canales J, Cabezas A, Cameselle J C

机构信息

Unidad de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Extremadura, Apartado 108, E-06080 Badajoz, Spain.

出版信息

Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):881-8. doi: 10.1042/bj3300881.

DOI:10.1042/bj3300881
PMID:9480905
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1219220/
Abstract

An enzyme activity splitting FAD to AMP and riboflavin 4',5'-cyclic phosphate (4',5'-cFMN), with a Km of 6-8 microM, was partially purified from the cytosolic fraction of rat liver homogenates. 4', 5'-cFMN was characterized by enzyme, HPLC, UV-visible and NMR spectroscopic analyses. The data suggest that a novel enzyme, tentatively named FAD-AMP lyase (cyclizing) or FMN cyclase, is involved. Also, 4',5'-cFMN was hydrolysed to 5'-FMN by a rat liver cyclic phosphodiesterase. The results indicate a novel enzymic pathway for flavins in mammals, and support the biological relevance of 4',5'-cFMN, perhaps as a flavocoenzyme or a regulatory signal.

摘要

从大鼠肝脏匀浆的胞质部分中部分纯化出一种酶活性，该酶可将黄素腺嘌呤二核苷酸（FAD）分解为腺苷酸（AMP）和核黄素4',5'-环磷酸酯（4',5'-cFMN），其米氏常数（Km）为6 - 8微摩尔。通过酶分析、高效液相色谱（HPLC）、紫外可见光谱和核磁共振（NMR）光谱分析对4',5'-cFMN进行了表征。数据表明，涉及一种新酶，暂命名为FAD - AMP裂解酶（环化）或FMN环化酶。此外，大鼠肝脏环磷酸二酯酶可将4',5'-cFMN水解为5'-FMN。结果表明哺乳动物体内黄素存在一种新的酶促途径，并支持4',5'-cFMN的生物学相关性，其可能作为黄素辅酶或调节信号。