PhD Program in Medical Sciences and CEMyQ, Universidad de La Frontera, Manuel Montt 112, Office 408, Temuco, Chile.
Center of Excellence in Morphological and Surgical Studies (CEMyQ), Universidad de La Frontera, Temuco, Chile.
Acta Parasitol. 2022 Mar;67(1):161-185. doi: 10.1007/s11686-021-00439-8. Epub 2021 Jul 15.
The aim of this study was to develop a synthesis of the evidence available regarding verified E. granulosus sensu lato (s.l.) genotypes in different species worldwide.
A systematic review was performed including studies concerning genotypes of E. granulosus s.l. without language or genotyped method restriction, published between 1990 and 2020. A systematic search was carried out in Trip Database, BIREME, SciELO, LILACS, IBECS, PAHO-WHO, EMBASE, PubMed, Scopus, and WoS. Variables of interest were year of publication, country, number of samples, and hosts; genotypes, molecular marker, haplotypes and molecular biology techniques used. Descriptive statistics were applied.
2411 articles were analyzed, however 135 met the selection criteria, representing 8643 liver and lung samples. Of the samples selected 24% were human, the remaining samples pertained to non-human animal hosts; cattle and sheep prevailed with 28.6% and 26.6% of the studied samples, respectively. The reported evidence is mainly from Iran, Turkey, Argentina, China and Chile; with 50, 11, 6, 6 and 5 studies, respectively, published between 1992 and 2020 [most frequently during 2015-2020 (76/135 studies; 56.3%)]. The mitochondrial gene cox1 was generally sequenced and informative (91.8%). Genotypes most frequently identified were E. granulosus sensu stricto (s.s.) (83.2%).
Based on this overall evidence, it can be concluded that publications related to genotypes of E. granulosus s.l. are heterogeneous. E. granulosus ss accounts for the vast majority of the global burden of E. granulosus s.l. worldwide. Further studies including larger number of cases and adequate internal validity are required to specify the distribution of genotypes in various host species.
PROSPERO CRD42018099827.
本研究旨在综合全球不同物种中已验证的细粒棘球绦虫（E. granulosus）s.l.基因型的现有证据。
本研究进行了系统综述，包括对细粒棘球绦虫 s.l. 基因型的研究，不限制语言或基因分型方法，发表时间为 1990 年至 2020 年。在 Trip 数据库、BIREME、SciELO、LILACS、IBECS、PAHO-WHO、EMBASE、PubMed、Scopus 和 WoS 中进行了系统检索。感兴趣的变量包括出版年份、国家、样本数量和宿主；使用的基因型、分子标记、单倍型和分子生物学技术。应用描述性统计。
分析了 2411 篇文章，然而，有 135 篇符合选择标准，代表了 8643 个肝和肺样本。所选样本中有 24%是人类样本，其余样本属于非人类动物宿主；牛和绵羊分别占研究样本的 28.6%和 26.6%。报告的证据主要来自伊朗、土耳其、阿根廷、中国和智利；分别有 50、11、6、6 和 5 项研究，发表时间在 1992 年至 2020 年之间[最常发表在 2015 年至 2020 年期间（76/135 项研究；56.3%）]。线粒体基因 cox1 通常被测序且具有信息性（91.8%）。最常鉴定的基因型是细粒棘球绦虫 s.s.（83.2%）。
根据这一总体证据，可以得出结论，与细粒棘球绦虫 s.l. 基因型相关的出版物是多样的。E. granulosus ss 占全球细粒棘球绦虫 s.l. 全球负担的绝大多数。需要进一步开展包括更多病例和适当内部有效性的研究，以确定各种宿主物种中基因型的分布。
PROSPERO CRD42018099827。
