Pacini F, Vivaldi A, Santoro M, Fedele M, Fusco A, Romei C, Basolo F, Pinchera A
Istituto di Endocrinologia, Metodologia Clinica e Medicina del Lavoro, Pisa, Italia.
Oncogene. 1998 Feb 5;16(5):665-9. doi: 10.1038/sj.onc.1201552.
Sequences of the SV40 virus, a virus of Asian macaques, have been found in human tumors, such as pleural mesotheliomas, ependimomas and choroid plexus tumors. Transgenic mice carrying the SV40 large T gene under the transcriptional control of the thyroglobulin gene promoter, develop thyroid dedifferentiation and follicular thyroid cell proliferation, leading to thyroid hyperplasia and adenocarcinomas. On these bases we investigated the presence of SV40 DNA sequences in 69 samples of papillary thyroid carcinomas (PTC) and in other thyroid and non-thyroid carcinomas, as well as in benign thyroid diseases. By Southern blot and PCR amplification followed by sequence analysis, we found the presence of SV40-related sequences integrated in the tumoral DNA of three cases of PTC. At least the 203 bp fragment of the aminoterminus of large T antigen, the 294 bp fragment of the VP1 gene and the 483 bp entire regulatory region were present in the tumoral DNA of these patients. SV40 sequences were not found in tissues other than PTC. Our results demonstrate that, in addition to previous findings in mesotheliomas and brain tumors, SV40 is somehow linked to papillary thyroid carcinoma. Although our data do not demonstrate a causative role in the development of PTC, this possibility must be considered and requires further studies.
在人类肿瘤中发现了亚洲猕猴病毒SV40的序列，这些肿瘤包括胸膜间皮瘤、室管膜瘤和脉络丛肿瘤。携带在甲状腺球蛋白基因启动子转录控制下的SV40大T基因的转基因小鼠，会发生甲状腺去分化和甲状腺滤泡细胞增殖，进而导致甲状腺增生和腺癌。基于这些，我们研究了69例甲状腺乳头状癌（PTC）样本以及其他甲状腺和非甲状腺癌样本以及良性甲状腺疾病样本中SV40 DNA序列的存在情况。通过Southern印迹法和PCR扩增，随后进行序列分析，我们发现3例PTC肿瘤DNA中存在与SV40相关的序列。这些患者的肿瘤DNA中至少存在大T抗原氨基末端的203 bp片段、VP1基因的294 bp片段和483 bp的整个调控区域。在PTC以外的组织中未发现SV40序列。我们的结果表明，除了先前在间皮瘤和脑肿瘤中的发现外，SV40在某种程度上与甲状腺乳头状癌有关。尽管我们的数据并未证明其在PTC发生中具有致病作用，但必须考虑这种可能性，并且需要进一步研究。
