Galateau-Salle F, Bidet P, Iwatsubo Y, Gennetay E, Renier A, Letourneux M, Pairon J C, Moritz S, Brochard P, Jaurand M C, Freymuth F
Department of Pathology, CHRU Caen, France.
Dev Biol Stand. 1998;94:147-52.
Mesotheliomas are pleural-based tumours mainly associated with asbestos exposure (70% of cases) and the incidence is still raising. Recently, a possible viral connection was reported and 60% of mesotheliomas were demonstrated to contain and express SV40-like DNA sequences. In this study, the presence of SV40-like DNA sequences were investigated in mesotheliomas (15 tissue samples and six cell lines) and in 63 additional bronchopulmonary carcinomas, one parietal osteosarcoma and non-malignant lung samples as well as in organizing pleuritis (8). Finally, 163 samples were analysed by the polymerase chain reaction (PCR) with a set of primers PYV.for and PYV.rev to amplify a 173 bp region of the SV40 Tag. and a 179 bp region JC virus (JCV) as well as a 182 bp region BK virus (BKV). PCR amplification and hybridization with a probe specific for SV40 Tag revealed that 47.6% of mesotheliomas, 28.6% of primary bronchopulmonary carcinomas and 16% of non-neoplasic lung diseases contained SV40-like DNA sequences. No statistically significant difference in the occurrence of these DNA sequences was found between malignant mesothelioma and bronchopulmonary carcinoma. However, a significantly higher number of mesothelioma cases exhibited SV40- like DNA sequences in comparison with non-malignant pleural and pulmonary tissues. The DNA sequences were not related to BK and JC virus sequences. These results indicate that SV40-like DNA sequences are present in mesotheliomas as well as in bronchopulmonary carcinomas and non-malignant pleuropulmonary diseases.
间皮瘤是主要基于胸膜的肿瘤，主要与石棉暴露有关（70%的病例），且发病率仍在上升。最近，有报道称可能存在病毒关联，并且已证实60%的间皮瘤含有并表达SV40样DNA序列。在本研究中，对间皮瘤（15个组织样本和6个细胞系）、另外63例支气管肺癌、1例骨膜骨肉瘤和非恶性肺组织样本以及机化性胸膜炎（8例）中的SV40样DNA序列进行了研究。最后，使用一组引物PYV.for和PYV.rev通过聚合酶链反应（PCR）对163个样本进行分析，以扩增SV40大T抗原的173 bp区域、JC病毒（JCV）的179 bp区域以及BK病毒（BKV）的182 bp区域。PCR扩增以及与针对SV40大T抗原的特异性探针杂交显示，47.6%的间皮瘤、28.6%的原发性支气管肺癌和16%的非肿瘤性肺部疾病含有SV40样DNA序列。在恶性间皮瘤和支气管肺癌之间，这些DNA序列的出现率没有统计学上的显著差异。然而，与非恶性胸膜和肺组织相比，间皮瘤病例中显示出SV40样DNA序列的数量明显更多。这些DNA序列与BK病毒和JC病毒序列无关。这些结果表明，SV40样DNA序列存在于间皮瘤、支气管肺癌以及非恶性胸膜肺疾病中。
