Katyal S L, Lombardi B L
Lipids. 1976 Jul;11(7):513-6. doi: 10.1007/BF02532895.
The contents of phosphatidyl N-methyl- and N,N-dimethylaminoethanol were determined in the liver of rats injected with (Me-C14) methionine. Total phospholipids were extracted from aliquots of the liver and fractionated by two-dimensional thin layer chromatography after addition of carrier phosphatidyl-N-methyl- and N,N-dimethylaminoethanol. The radioactivity present in the two phosphatide spots was determined and used to calculate total disintegrations per min/100 g body wt. The remainder of the livers was pooled, and total phospholipids were isolated and subjected to acid hydrolysis. N-methyl- and N,N-dimethylaminoethanol were purified by thin layer chromatography, and their specific activity was determined after quantitation by gas liquid chromatography and radioactivity measurement. The liver contents of phosphatidyl N-methyl- and N,N-dimethylaminoethanol were determined by dividing disintegrations per min/100 g body wt by the specific activity of N-methyl- or N,N-dimethylaminoethanol.
测定了注射（甲基 - C14）蛋氨酸的大鼠肝脏中磷脂酰 N - 甲基 - 和 N，N - 二甲基氨基乙醇的含量。从肝脏等分试样中提取总磷脂，并在加入载体磷脂酰 - N - 甲基 - 和 N，N - 二甲基氨基乙醇后通过二维薄层色谱法进行分离。测定两个磷脂斑点中的放射性，并用于计算每分钟每 100 克体重的总衰变次数。将其余的肝脏合并，分离出总磷脂并进行酸水解。通过薄层色谱法纯化 N - 甲基 - 和 N，N - 二甲基氨基乙醇，并在通过气相色谱法定量和放射性测量后测定其比活性。通过将每分钟每 100 克体重的衰变次数除以 N - 甲基 - 或 N，N - 二甲基氨基乙醇的比活性来测定肝脏中磷脂酰 N - 甲基 - 和 N，N - 二甲基氨基乙醇的含量。
