免疫球蛋白突变体依赖于大型内含子增强子的存在、位置和方向。
The Ig mutator is dependent on the presence, position, and orientation of the large intron enhancer.
作者信息
Bachl J, Olsson C, Chitkara N, Wabl M
机构信息
Basel Institute for Immunology, Postfach, CH-4005 Basel, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2396-9. doi: 10.1073/pnas.95.5.2396.
Abstract
Hypermutation at the Ig loci is confined to the area between the promoter and the intronic enhancer, which includes the rearranged variable region gene segment. We identified factors that contribute to the site-specificity at the heavy chain locus. We found that distance from both the promoter and the intronic enhancer is crucial in hypermutation. The presence of the enhancer is required, and, in contrast to its definition for transcriptional activity, its effect is orientation-sensitive.
摘要
免疫球蛋白基因座的高突变局限于启动子和内含子增强子之间的区域，该区域包括重排的可变区基因片段。我们鉴定了促成重链基因座位点特异性的因素。我们发现，与启动子和内含子增强子的距离在高突变中至关重要。增强子的存在是必需的，并且与其对转录活性的定义相反，其作用是方向敏感的。
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Modifying the sequence of an immunoglobulin V-gene alters the resulting pattern of hypermutation.改变免疫球蛋白V基因的序列会改变由此产生的高突变模式。
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