Staurenghi A H, Masera R G, Prolo P, Griot G, Sartori M L, Ravizza L, Angeli A
Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Azienda Ospedaliera S. Luigi, Orbassano, Italy.
Psychoneuroendocrinology. 1997 Nov;22(8):575-90. doi: 10.1016/s0306-4530(97)00070-x.
To evaluate the role of Hypothalamic-Pituitary-Adrenal (HPA) hormones and psychoneuroendocrine modulation on NK cell activity in Anorexia Nervosa (AN) we studied in 24 patients and 20 sex- and age-matched healthy controls, the spontaneous NK activity of peripheral blood mononuclear (PBM) cells and the susceptibility in vitro to cortisol or immune interferon or interleukin-2. NK cytotoxicity of PBM cells was measured in a direct non-radiometric 4h cytolytic assay using K562 cells as targets. HPA axis function was evaluated by IV ovine Corticotropin Releasing Hormone (o-CRH) administration. We did not find clear-cut abnormalities of NK cytotoxicities either in basal conditions or after exposure to challengers. The extent of cortisol-dependent inhibition was comparable in patients and controls. Significant inverse and direct correlations were found respectively between the spontaneous NK cell activity and baseline serum cortisol at 0800 h (r = -0.5; p < .02), and between IL-2 dependent boosting of NK cell cytotoxicity and ACTH, beta-endorphin or cortisol responses after o-CRH, expressed as areas under the curve (AUC) (r = 0.46, p < .05; r = 0.46, p < .05; and r = -0.48, p < .05, respectively). Correlations observed with AUC ratios yielded more significant results (r = 0.62; p < .01 and r = 0.51; p < .05 respectively). These data suggest a role for Proopiomelanocortin (POMC) derived peptides in the regulation of NK cell activity in AN, and multifaceted relationships between this particular immune function, on the one hand, and certain patterns of HPA axis function on the other.
为评估下丘脑 - 垂体 - 肾上腺（HPA）激素及精神神经内分泌调节在神经性厌食症（AN）中对自然杀伤（NK）细胞活性的作用，我们研究了24例患者及20名性别和年龄匹配的健康对照者，检测外周血单个核（PBM）细胞的自发NK活性以及体外对皮质醇、免疫干扰素或白细胞介素 - 2的敏感性。使用K562细胞作为靶细胞，通过直接非放射性4小时细胞溶解试验测量PBM细胞的NK细胞毒性。通过静脉注射羊促肾上腺皮质激素释放激素（o - CRH）评估HPA轴功能。我们未发现基础状态下或接触激发剂后NK细胞毒性有明确异常。患者和对照者中皮质醇依赖性抑制程度相当。分别在自发NK细胞活性与08:00时的基线血清皮质醇之间（r = -0.5；p <.02），以及IL - 2依赖性增强的NK细胞毒性与o - CRH后促肾上腺皮质激素（ACTH）、β - 内啡肽或皮质醇反应（以曲线下面积（AUC）表示）之间（r = 0.46，p <.05；r = 0.46，p <.05；r = -0.48，p <.05）发现显著的负相关和正相关。与AUC比值的相关性产生了更显著的结果（分别为r = 0.62；p <.01和r = 0.51；p <.05）。这些数据表明阿片 - 促黑素 - 皮质素原（POMC）衍生肽在AN中NK细胞活性调节中起作用，以及一方面这种特定免疫功能与另一方面某些HPA轴功能模式之间存在多方面关系。
