Ichiyama T, Nishikawa M, Yoshitomi T, Hayashi T, Furukawa S
Department of Pediatrics, Yamaguchi University School of Medicine, Japan.
Neurology. 1998 Feb;50(2):407-11. doi: 10.1212/wnl.50.2.407.
We investigated the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1 beta), and IL-6 as proinflammatory cytokines in CSF to facilitate differentiation between acute encephalitis/encephalopathy and prolonged febrile seizures. We studied 20 children with prolonged febrile seizures and 23 with acute encephalitis/encephalopathy, including 8 with an acellular CSF. TNF-alpha, IL-1 beta, and IL-6 in CSF were measured by ELISA. We found that TNF-alpha, IL-1 beta, and IL-6 were undetectable in CSF of all children with prolonged febrile seizures and control subjects but that the concentrations of TNF-alpha was elevated in 11, of IL-1 alpha in 6, and of IL-6 in 17 of 23 children with acute encephalitis/encephalopathy. Twenty-two of 23 children with acute encephalitis/encephalopathy had elevated concentrations of one or more cytokine. Elevated concentrations of the CSF proinflammatory cytokines, TNF-alpha, IL-1 beta, and IL-6, indicate acute encephalitis/encephalopathy rather than febrile seizures.
我们检测了脑脊液中作为促炎细胞因子的肿瘤坏死因子-α（TNF-α）、白细胞介素-1α（IL-1β）和IL-6的水平，以有助于鉴别急性脑炎/脑病和长时间热性惊厥。我们研究了20例长时间热性惊厥患儿和23例急性脑炎/脑病患儿，其中8例脑脊液无细胞。采用酶联免疫吸附测定法（ELISA）检测脑脊液中的TNF-α、IL-1β和IL-6。我们发现，所有长时间热性惊厥患儿和对照受试者的脑脊液中均未检测到TNF-α、IL-1β和IL-6，但在23例急性脑炎/脑病患儿中，11例TNF-α浓度升高，6例IL-1α浓度升高，17例IL-6浓度升高。23例急性脑炎/脑病患儿中有22例一种或多种细胞因子浓度升高。脑脊液促炎细胞因子TNF-α、IL-1β和IL-6浓度升高表明为急性脑炎/脑病而非热性惊厥。
