Hauschild K, Pauli H H, Kutchan T M
Laboratorium für Molekulare Biologie, Universität München, Germany.
Plant Mol Biol. 1998 Feb;36(3):473-8. doi: 10.1023/a:1005917808232.
A genomic clone, bbe1 was isolated that encodes the methyl jasmonate-inducible berberine bridge enzyme of antimicrobial benzophenanthridine alkaloid biosynthesis in the California poppy Eschscholzia californica. Genomic DNA gel blot analysis indicates that two genes are present in the E. californica genome that code for the berberine bridge enzyme reading frame. Each coding region is apparently preceeded by a unique promoter sequence. The bbe1 gene contains no introns and one transcriptional start site. A 41 nucleotide region between -496 and -455 of the 5'-flanking region appears to be essential for promoter activity in E. californica. The promoter displayed an unexpectedly high species specificity, being active in only E. californica and Thalictrum bulgaricum, out of 28 cell suspension cultures tested.
分离出一个基因组克隆bbe1，它编码加利福尼亚罂粟（Eschscholzia californica）中抗菌苯并菲啶生物碱生物合成的茉莉酸甲酯诱导型小檗碱桥酶。基因组DNA凝胶印迹分析表明，加利福尼亚罂粟基因组中存在两个编码小檗碱桥酶阅读框的基因。每个编码区之前显然都有一个独特的启动子序列。bbe1基因不含内含子，有一个转录起始位点。5'侧翼区-496至-455之间的一个41核苷酸区域似乎对加利福尼亚罂粟中的启动子活性至关重要。在测试的28种细胞悬浮培养物中，该启动子表现出出乎意料的高物种特异性，仅在加利福尼亚罂粟和保加利亚唐松草中具有活性。
