Gundlach H, Müller M J, Kutchan T M, Zenk M H
Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2389-93. doi: 10.1073/pnas.89.6.2389.
To deter pathogenic microorganisms and herbivores, plants have developed an inducible chemical defense system. It is known that the induced synthesis of low molecular weight compounds can be provoked by exposing cultured cells to fungal cell wall fragments. In this study we show that endogenous jasmonic acid and its methyl ester accumulate rapidly and transiently after treatment of plant cell suspension cultures of Rauvolfia canescens and Eschscholtzia californica with a yeast elicitor. Thirty-six plant species tested in cell suspension culture could be elicited with respect to the accumulation of secondary metabolites by exogenously supplied methyl jasmonate. Addition of methyl jasmonate initiates de novo transcription of genes, such as phenylalanine ammonia lyase, that are known to be involved in the chemical defense mechanisms of plants. These data demonstrate the integral role of jasmonic acid and its derivatives in the intracellular signal cascade that begins with interaction of an elicitor molecule with the plant cell surface and results, ultimately, in the accumulation of secondary compounds.
为了抵御病原微生物和食草动物，植物已经进化出一种可诱导的化学防御系统。已知通过将培养细胞暴露于真菌细胞壁片段可以激发低分子量化合物的诱导合成。在本研究中，我们表明，用酵母激发子处理萝芙木和加州罂粟的植物细胞悬浮培养物后，内源茉莉酸及其甲酯会迅速且短暂地积累。在细胞悬浮培养中测试的36种植物物种，通过外源供应茉莉酸甲酯，可以诱导其积累次生代谢产物。添加茉莉酸甲酯会启动基因的从头转录，例如苯丙氨酸解氨酶，这些基因已知参与植物的化学防御机制。这些数据证明了茉莉酸及其衍生物在细胞内信号级联反应中的不可或缺的作用，该信号级联反应始于激发子分子与植物细胞表面的相互作用，并最终导致次生化合物的积累。
