Dekumyoy P, Waikagul J, Eom K S
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Trop Med Int Health. 1998 Jan;3(1):52-6. doi: 10.1046/j.1365-3156.1998.00172.x.
The specificity of three major polypeptides (35, 33 and 32.5 kD) from Paragonimus heterotremus antigens prepared from ether-extracted adult worms was tested against sera from heterologous infections as well as against P. westermani-infected sera. Only the 35 kD polypeptide was not present, its antigenic determinant being bound to the antibodies from all P. westermani-infected cases. Its cross-reactivity against various sera from heterologous helminthiases and other lung infections showed that it is not bound to these antigenic polypeptides. These major bands cannot be detected by Concanavalin A detector. Our research encourages the pattern (35, 33 and 32.5 kD) of immunoblot reactions for the diagnosis of P. heterotremus infections; the 35 kD antigen is specific for corresponding species and able to differentiate infections between both species of Paragonimus.
对从经乙醚提取的异盘并殖吸虫成虫制备的抗原中三种主要多肽（35、33和32.5 kD）的特异性进行了检测，检测对象为来自异种感染的血清以及来自卫氏并殖吸虫感染的血清。只有35 kD多肽不存在，其抗原决定簇与所有卫氏并殖吸虫感染病例的抗体结合。它对来自异种蠕虫病和其他肺部感染的各种血清的交叉反应表明，它不与这些抗原多肽结合。这些主要条带不能用伴刀豆球蛋白A检测器检测到。我们的研究支持将免疫印迹反应模式（35、33和32.5 kD）用于异盘并殖吸虫感染的诊断；35 kD抗原对相应物种具有特异性，能够区分两种并殖吸虫之间的感染。
