Intapan Pewpan M, Sanpool Oranuch, Janwan Penchom, Laummaunwai Porntip, Morakote Nimit, Kong Yoon, Maleewong Wanchai
Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Korean J Parasitol. 2013 Dec;51(6):763-6. doi: 10.3347/kjp.2013.51.6.763. Epub 2013 Dec 31.
A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.
基于卫氏并殖吸虫组织蛋白酶L前体原的抗原区域制备了一种合成肽，并使用酶联免疫吸附测定（ELISA）检测患者血清中的IgG4抗体，测试其对人体并殖吸虫病（由异盘并殖吸虫引起）免疫诊断的适用性。来自其他蠕虫病、结核病患者的血清以及健康志愿者的血清用作对照。这种基于肽的检测系统的灵敏度、特异性、准确性、阳性预测值和阴性预测值分别为100%、94.6%、96.2%、100%和88.9%。经常观察到该检测系统与姜片虫病患者血清（75%）和钩虫感染患者血清（50%）发生交叉反应。由于并殖吸虫病和姜片虫病的鉴别诊断可以通过临床表现和姜片虫病血清学轻松完成，因此这种交叉反应不是一个严重问题。其他寄生虫病患者的血清（0 - 25%）很少对这种合成抗原产生反应。这种合成肽抗原似乎有助于开发一种标准化的并殖吸虫病诊断系统。
