Akerman K K, Jolkkonen J, Huttunen H, Penttilä I
Department of Clinical Chemistry, Kuopio University Hospital, Finland.
Ther Drug Monit. 1998 Feb;20(1):25-9. doi: 10.1097/00007691-199802000-00005.
This report describes a sensitive and specific method for analyzing a serotonin reuptake blocker, citalopram, and its active metabolite, desmethylcitalopram, in human serum. For high-performance liquid chromatography (HPLC) analysis, samples and standards are prepared with ASPEC automatic sample preparator using 100 mg Bond-Elut C-18 solid-phase extraction columns. The method is an isocratic HPLC method with a mobile phase of acetonitrile:methanol:50 mM dipotassium hydrogenphosphate, pH 4.7 (40:100). Detection is performed with diode array detector at 220 nm and the peak purity analyses at 210 to 365 nm. The intraassay coefficient of variation ranges from 3.7% to 7.3%, and the interassay coefficient of variation ranges from 6.9% to 9.9% at therapeutic drug concentrations. The detection limit is 15 nmol/l. The method is suitable for therapeutic drug monitoring in a clinical laboratory. A clear correlation, r = 0.72 (y = 0.36x + 17.94), between citalopram and its metabolite levels is observed in routine therapeutic drug monitoring service. A linear correlation between serum concentration and daily dose of citalopram in patient groups is also observed.
本报告描述了一种灵敏且特异的方法，用于分析人血清中的5-羟色胺再摄取阻滞剂西酞普兰及其活性代谢物去甲西酞普兰。对于高效液相色谱（HPLC）分析，使用100mg Bond-Elut C-18固相萃取柱，通过ASPEC自动样品制备仪制备样品和标准品。该方法为等度HPLC法，流动相为乙腈：甲醇：50mM磷酸氢二钾，pH 4.7（40:100）。使用二极管阵列检测器在220nm处进行检测，并在210至365nm处进行峰纯度分析。在治疗药物浓度下，批内变异系数范围为3.7%至7.3%，批间变异系数范围为6.9%至9.9%。检测限为15nmol/l。该方法适用于临床实验室的治疗药物监测。在常规治疗药物监测服务中，观察到西酞普兰与其代谢物水平之间存在明显的相关性，r = 0.72（y = 0.36x + 17.94）。在患者组中，还观察到血清浓度与西酞普兰日剂量之间存在线性相关性。
