Olesen O V, Linnet K
Department of Biological Psychiatry, Psychiatric Hospital, Aarhus University Hospital, Risskov, Denmark.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):83-8. doi: 10.1016/0378-4347(95)00347-9.
A simplified method for the determination of racemic citalopram and its main metabolite desmethylcitalopram in serum using HPLC was developed. The compounds were extracted with heptane-isoamyl alcohol (98:2) and subsequently transferred into phosphate buffer pH 2.5 for direct injection into the HPLC apparatus. The analytes were separated with an acetonitrile-phosphate buffer, pH 2.5-tetraethylamine mobile phase on a C18 column and measured by UV detection at 240 nm. Within the typical range of serum concentrations (30-100 ng/ml) the inter-day variation was < 6% for both compounds. Possible analytical interference from a number of commonly coadministered psychoactive drugs and their metabolites was studied by extracting sera from patients receiving these drugs. Interference was not a problem for the developed method.
开发了一种使用高效液相色谱法（HPLC）测定血清中消旋西酞普兰及其主要代谢物去甲基西酞普兰的简化方法。化合物用庚烷 - 异戊醇（98:2）萃取，随后转移至pH 2.5的磷酸盐缓冲液中，直接注入HPLC仪器。分析物在C18柱上用乙腈 - pH 2.5的磷酸盐缓冲液 - 四乙胺流动相进行分离，并通过在240 nm处的紫外检测进行测定。在血清浓度的典型范围内（30 - 100 ng/ml），两种化合物的日间变化均<6%。通过萃取接受这些药物治疗的患者的血清，研究了多种常用的共同给药精神活性药物及其代谢物可能产生的分析干扰。对于所开发的方法，干扰不是问题。
