Schuppe H C, Kulig J, Lerchenmüller C, Becker D, Gleichmann E, Kind P
Department of Dermatology, Heinrich Heine University, Düsseldorf, Germany.
Toxicol Lett. 1997 Dec;93(2-3):125-33. doi: 10.1016/s0378-4274(97)00085-4.
Complex platinum (Pt) compounds are known as occupational respiratory sensitizers whereas their role in skin exposure is unclear. In this study, both skin irritation and induction of contact hypersensitivity by halide Pt salts were characterized in mice. Repeated application of Na2[PtCl6] (5% in acetone) to both ears of naive BALB/c mice induced activation of the draining auricular lymph nodes. Flow cytometric analysis revealed a striking increase in the number of lymph node cells expressing proliferating cell nuclear antigen. In separate experiments, Na2[PtCl6] or acetone were applied only to the right ear of mice on 4-8 consecutive days and the animals were challenged on the left ear 6 days later. Ear thickness was determined before and 0.5, 24, 48, and 72 h after challenge with 0.5 or 2% Na2[PtCl6] or acetone. Maximal swelling of the left ear was recorded at 48 h in Pt-sensitized mice challenged with 2% Na2[PtCl6]. Furthermore, the concentration of Na2[PtCl6] required for sensitization caused an irritant reaction as demonstrated by significant swelling of the right ear. These data support the concept that both irritant and allergic contact dermatitis to halide Pt salts may occur in humans. Concerning skin exposure to halide Pt salts, Pt-induced irritant reactions resulting from an intrinsic adjuvant's activity of the compound could be a prerequisite for sensitization.
复杂的铂（Pt）化合物被认为是职业性呼吸道致敏剂，但其在皮肤接触中的作用尚不清楚。在本研究中，对卤化铂盐在小鼠中的皮肤刺激性和接触性超敏反应诱导进行了表征。将Na2[PtCl6]（5%溶于丙酮）反复涂抹于未接触过的BALB/c小鼠的双耳，可诱导引流耳淋巴结活化。流式细胞术分析显示，表达增殖细胞核抗原的淋巴结细胞数量显著增加。在单独的实验中，连续4-8天将Na2[PtCl6]或丙酮仅涂抹于小鼠的右耳，6天后对左耳进行激发。在用0.5%或2%的Na2[PtCl6]或丙酮激发前及激发后0.5、24、48和72小时测定耳厚度。在用2%的Na2[PtCl6]激发的铂致敏小鼠中，左耳在48小时时出现最大肿胀。此外，致敏所需的Na2[PtCl6]浓度会引起刺激反应，如右耳明显肿胀所示。这些数据支持了卤化铂盐在人类中可能同时引起刺激性和过敏性接触性皮炎的概念。关于皮肤接触卤化铂盐，该化合物固有的佐剂活性导致的铂诱导的刺激反应可能是致敏的先决条件。
