Stringer C P, Hicks R, Botham P A
ICI, Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Contact Dermatitis. 1991 Mar;24(3):210-5. doi: 10.1111/j.1600-0536.1991.tb01700.x.
Contact sensitivity (allergic contact dermatitis) to bis(tri-n-butyltin) oxide (TBTO) was demonstrated in the mouse. TBTO (in an acetone:olive olive oil vehicle) or acetone:olive oil alone (as a control) were applied to the shaved flank under an occlusive patch and animals were challenged on the dorsum of the ear 6 days later. Ear swelling was then measured using an engineer's micrometer, 2, 24, 48 or 72 h thereafter. Significant differences in ear swelling between control and TBTO-sensitized animals were found 24 h after challenge: thereafter the elicitation reaction declined rapidly whilst irritant swelling in control animals increased. Maximal elicitation of TBTO sensitivity could only be elicited by concentrations of TBTO that caused irritation. In a separate experiment, a single application of TBTO to the ears of naive animals provoked increase in auricular lymph node weight, cell yield and proliferation of lymph node cells during overnight culture. These data support the conclusion that TBTO is a contact sensitizer and illustrate the potential usefulness of the quantitative methods of contact sensitivity assessment which have been developed in the mouse.
在小鼠身上证实了对双（三正丁基锡）氧化物（TBTO）的接触性敏感（过敏性接触性皮炎）。将TBTO（溶于丙酮：橄榄油载体中）或仅丙酮：橄榄油（作为对照）涂于剃毛后的胁腹并覆盖封闭性贴片，6天后在动物耳部背面进行激发。然后在激发后2、24、48或72小时使用工程师用测微计测量耳部肿胀情况。激发后24小时发现对照动物和TBTO致敏动物的耳部肿胀存在显著差异：此后激发反应迅速下降，而对照动物的刺激性肿胀增加。只有引起刺激的TBTO浓度才能引发TBTO敏感性的最大激发。在另一项实验中，将TBTO单次涂于未接触过的动物耳部，会在过夜培养期间引起耳淋巴结重量增加、细胞产量增加以及淋巴结细胞增殖。这些数据支持了TBTO是一种接触性致敏剂的结论，并说明了在小鼠中开发的接触性敏感评估定量方法的潜在实用性。
