[E-test评估：唑类抗真菌药敏试验中E-test与微量肉汤稀释法的比较]

[Evaluation of the E-test: comparison of E-test and microbroth dilution method in azole antifungal susceptibility test].

作者信息

Komaki H, Tanaka Y, Yazawa K, Kadowaki K, Thomson K S, Mikami Y

机构信息

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.

出版信息

Nihon Ishinkin Gakkai Zasshi. 1998;39(1):27-31. doi: 10.3314/jjmm.39.27.

DOI:10.3314/jjmm.39.27

PMID:9487005

Abstract

The susceptibilities of clinical isolates of Candida spp. to fluconazole (FLCZ), itraconazole (ITCZ) and ketoconazole (KCZ) were determined by a new method, E-test (AB BIODISK, Sweden) and compared with those obtained by the microbroth dilution method. The MIC values of the 3 azoles obtained by the two methods were, in general, in good agreement. While MICs of FLCZ and KCZ demonstrated higher essential agreement rates and better correlation by both methods, MIC values of ITCZ by the E-test had a tendency to be higher than those of the microbroth dilution method in azole resistant strains. Potential usability of E-test as a broth dilution method for susceptibility tests for clinically isolated Candida spp. including azole resistant strains was discussed.

摘要

采用一种新方法——E-test（瑞典AB BIODISK公司）测定了念珠菌属临床分离株对氟康唑（FLCZ）、伊曲康唑（ITCZ）和酮康唑（KCZ）的敏感性，并与微量肉汤稀释法的结果进行了比较。两种方法获得的3种唑类药物的MIC值总体上吻合良好。虽然两种方法测定的FLCZ和KCZ的MIC显示出更高的基本符合率和更好的相关性，但在唑类耐药菌株中，E-test测定的ITCZ的MIC值往往高于微量肉汤稀释法。讨论了E-test作为包括唑类耐药菌株在内的临床分离念珠菌属药敏试验肉汤稀释法的潜在实用性。