Ntanios F Y, Jones P J
School of Dietetics and Human Nutrition, McGill University, MacDonald Campus 21, 171 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, Canada.
Biochim Biophys Acta. 1998 Feb 23;1390(3):237-44. doi: 10.1016/s0005-2760(97)00196-3.
To examine how variable sitostanol (SI) levels in phytosterol-supplemented diets influence plasma and hepatic lipid concentrations, fifty hamsters were divided into five groups and fed semipurified diets containing 0.25% (w/w) cholesterol for 45 days ad libitum. Four groups were fed this diet with 1% (w/w) phytosterol mixtures which contained 0.01% (w/w) SI derived from soybean, 0.2% (w/w) SI derived from tall oil, 0.2% (w/w) synthetic SI mixture (SIM) and 1% (w/w) pure SI, respectively. A control group did not receive phytosterols. Dietary SI supplementation at 1% (w/w) decreased total and non-apolipoprotein-A cholesterol levels in plasma by 34% (P=0.001) and 55% (P=0.04), respectively, whereas mean plasma total cholesterol level in the 0.2% (w/w) SI group was 23% (P=0.001) lower than that of the control group. Conversely, plasma lipid profile in hamsters fed 1 or 0.2% (w/w) SI did not differ from the 0.01% (w/w) SI group. Liver weights were 15 and 20% (P=0.012) higher in the control group compared with those fed 0.01% and 1% (w/w) SI, respectively, while the hepatic cholesterol content in the control group was greater (P<0.0001) than that of all other groups. Plasma campesterol levels were higher (P=0.04) in the 0.01% and 0.2% (w/w) SI fed groups than in the control, 0.2% (w/w) SIM and 1% (w/w) SI groups. Hepatic sitosterol content was elevated (P=0.002) in the SIM fed group compared to other groups. We conclude that dietary SI effect is proportional to its concentration in phytosterol mixtures and in the diet. Dietary SI lowered plasma cholesterol levels at concentrations higher than 0.2% (w/w) in hamsters. (c) 1998 Elsevier Science B.V.
为研究补充植物甾醇的饮食中，不同水平的谷甾烷醇（SI）如何影响血浆和肝脏脂质浓度，将50只仓鼠分为五组，随意喂食含0.25%（w/w）胆固醇的半纯化饮食45天。四组分别喂食含1%（w/w）植物甾醇混合物的这种饮食，这些混合物分别含有0.01%（w/w）源自大豆的SI、0.2%（w/w）源自妥尔油的SI、0.2%（w/w）合成SI混合物（SIM）和1%（w/w）纯SI。一个对照组未接受植物甾醇。饮食中补充1%（w/w）的SI可使血浆中总胆固醇和非载脂蛋白 - A胆固醇水平分别降低34%（P = 0.001）和55%（P = 0.04），而0.2%（w/w）SI组的平均血浆总胆固醇水平比对照组低23%（P = 0.001）。相反，喂食1%或0.2%（w/w）SI的仓鼠的血浆脂质谱与0.01%（w/w）SI组无差异。与喂食0.01%和1%（w/w）SI的仓鼠相比，对照组的肝脏重量分别高15%和20%（P = 0.012），而对照组的肝脏胆固醇含量高于所有其他组（P < 0.0001）。喂食0.01%和0.2%（w/w）SI的组的血浆菜油甾醇水平高于对照组、0.2%（w/w）SIM组和1%（w/w）SI组（P = 0.04）。与其他组相比，喂食SIM的组的肝脏甾醇含量升高（P = 0.002）。我们得出结论，饮食中SI的作用与其在植物甾醇混合物和饮食中的浓度成正比。在仓鼠中，饮食中SI浓度高于0.2%（w/w）时可降低血浆胆固醇水平。（c）1998爱思唯尔科学出版社B.V.
