柑橘属和枳属物种及其杂种的染色体：利用原位杂交和荧光带型鉴定特征染色体及rDNA位点的物理图谱

The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.

作者信息

Roose M L, Schwarzacher T, Heslop-Harrison J S

机构信息

Department of Cell Biology, John Innes Centre, Norwich, England.

出版信息

J Hered. 1998 Jan-Feb;89(1):83-6. doi: 10.1093/jhered/89.1.83.

DOI:10.1093/jhered/89.1.83

PMID:9487679

Abstract

In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x = 18), Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus x Poncirus hybrids (2n = 2x = 18). Counterstaining with the fluorochromes chromomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus x Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessions examined.

摘要

采用生物素或罗丹明标记的18S - 5.8S - 25S rDNA探针以及地高辛标记的5S rDNA探针原位杂交，以定位甜橙（Citrus sinensis L.，2n = 2x = 18）、枳（Poncirus trifoliata L. Raf.，2n = 2x = 18）和柑橘与枳的杂种（2n = 2x = 18）根尖中期染色体上的rDNA位点。用荧光染料色霉素A3和DAPI复染，能独特地识别许多但并非所有染色体。甜橙有5个18S - 25S rDNA位点，枳有7个，3个不同的柑橘与枳杂种有5个或6个位点。检测到4个5S rDNA位点，大多与18S - 25S rDNA位点相连。总体而言，我们在所检测的所有材料中都观察到了高水平的染色体杂合性。