Eaves-Pyles T, Alexander J W
University of Cincinnati Medical Center, Department of Surgery, Ohio 45267, USA.
Shock. 1998 Feb;9(2):95-100. doi: 10.1097/00024382-199802000-00004.
Loss of gut barrier function after burn injury can be important in the pathogenesis of systemic infections and organ dysfunction. The purpose of this study was to determine how rapidly impairment of gut barrier function occurs after burn injury and how long it persists. BALB/c mice were gavaged with 10(10) (111)In-oxine-labeled Escherichia coli 3 h before inflicting a 20% total body surface area burn. They were then killed at 5, 15, 30, 60, 120, or 240 min post-burn. Additional mice were given a 20% or 30% burn injury and were randomized into eight groups, which were killed at either 4 h or 1, 2, 4, 7, 14, or 21 days post-burn. Each mouse was gavaged with 10(10) (111)In-oxine-labeled E. coli 4 h before sacrifice to determine the magnitude of translocation. Gut barrier function was impaired as early as 5 min post-burn and was maximal by 4 h. Rapid improvement was observed by 24 h, followed by slow improvement, but with persistent abnormality through 21 days post-burn. Killing of translocated bacteria was impaired at 4 h and day 7 post-burn, according to the percentage of viable E. coli that remained alive in the tissues. The magnitude of gut dysfunction following burn injury is temporally related.
烧伤后肠道屏障功能丧失在全身感染和器官功能障碍的发病机制中可能起重要作用。本研究的目的是确定烧伤后肠道屏障功能损害多快出现以及持续多久。在造成20%体表面积烧伤前3小时，给BALB/c小鼠灌胃10(10) （111）铟-奥克辛标记的大肠杆菌。然后在烧伤后5、15、30、60、120或240分钟处死它们。另外的小鼠给予20%或30%的烧伤，并随机分为八组，在烧伤后4小时或1、2、4、7、14或21天处死。在处死前4小时给每只小鼠灌胃10(10) （111）铟-奥克辛标记的大肠杆菌，以确定移位的程度。肠道屏障功能在烧伤后5分钟就开始受损，4小时时达到最大。在24小时观察到快速改善，随后是缓慢改善，但在烧伤后21天仍存在持续异常。根据组织中存活的大肠杆菌的百分比，在烧伤后4小时和第7天，对移位细菌的杀灭受损。烧伤后肠道功能障碍的程度与时间有关。
