Alexander J W, Gianotti L, Pyles T, Carey M A, Babcock G F
Department of Surgery, University of Cincinnati Medical Center, Ohio 45267-0558.
Ann Surg. 1991 Jun;213(6):558-66; discussion 566-7. doi: 10.1097/00000658-199106000-00005.
The present investigation was performed to study the kinetics of tissue distribution and deposition of Escherichia coli and endotoxin translocating from the intestine after thermal injury. Escherichia coli was grown in the presence of 14C glucose and both labeled bacteria and endotoxin prepared from the labeled bacteria were used as translocation probes. Escherichia coli (10(8) to 10(10) bacteria) and E. coli endotoxin (100 micrograms per animal) were gavaged into the stomach immediately before a 30% burn injury was inflicted in mice. Animals were killed 1, 4 and 24 hours after burn injury. Translocation occurred extensively within 1 hour after burn injury. Expressed as amount of radioactivity per gram of tissue, translocation was greatest in the mesenteric lymph node (MLN) followed by spleen, lung, and liver. Translocation of endotoxin was similar to translocation of intact bacteria, with the exception that less radioactivity could be found in the peritoneal cavity and more in the liver. Both intact E. coli and endotoxin translocated directly through the intact bowel wall. Killing of bacteria was greatest in the MLN and spleen, approximating 95% to more than 99% of translocating bacteria. Killing efficiency was lowest in the lungs. It is concluded that estimation of translocation by viable bacterial counts in tissues grossly underestimates the extent of translocation of bacteria and ignores the extent of translocation of endotoxin. Translocation of endotoxin may have biologic significance that is independent of and in addition to translocation of intact bacteria.
本研究旨在探讨热损伤后从肠道移位的大肠杆菌和内毒素在组织中的分布及沉积动力学。大肠杆菌在含(^{14}C)葡萄糖的环境中生长，用标记的细菌及从标记细菌制备的内毒素作为移位探针。在对小鼠造成(30%)烧伤损伤前，立即将大肠杆菌（(10^8)至(10^{10})个细菌）和大肠杆菌内毒素（每只动物(100)微克）灌胃至胃内。在烧伤后(1)小时、(4)小时和(24)小时处死动物。烧伤后(1)小时内广泛发生移位。以每克组织的放射性活度表示，移位在肠系膜淋巴结（MLN）中最大，其次是脾脏、肺和肝脏。内毒素的移位与完整细菌的移位相似，不同之处在于腹腔内放射性活度较少，而肝脏中较多。完整的大肠杆菌和内毒素均直接穿过完整的肠壁。细菌在MLN和脾脏中的杀灭率最高，约为移位细菌的(95%)至超过(99%)。肺部的杀灭效率最低。结论是，通过组织中活菌计数估计移位严重低估了细菌移位的程度，并且忽略了内毒素的移位程度。内毒素的移位可能具有独立于完整细菌移位之外的生物学意义。
