Anderson K L, Megehee J A, Varel V H
Department of Biological Sciences, Mississippi State University, MS 39762, USA.
Lett Appl Microbiol. 1998 Jan;26(1):35-7. doi: 10.1046/j.1472-765x.1998.00265.x.
Tn1545, a self-mobilizing transposon, was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of 1 per 10(6) recipient cells. Transconjugants of Eu. cellulosolvens were resistant to both tetracycline and erythromycin, and were able to mobilize Tn1545 back into Cl. beijerinckii. Southern blot hybridization of representative transconjugants did not reveal site-specific insertion. This potential randomness of the transposon insertion site may prove useful in the development of Tn1545 as a tool for mutagenesis of Eu. cellulosolvens.
Tn1545是一种自我转移转座子，已被导入瘤胃纤维素分解菌溶纤维真杆菌的染色体中。这是通过将转座子从拜氏梭菌进行接合转移实现的，转移频率为每10^6个受体细胞中有1个。溶纤维真杆菌的接合子对四环素和红霉素都有抗性，并且能够将Tn1545再次转移回拜氏梭菌中。代表性接合子的Southern杂交未显示出位点特异性插入。转座子插入位点的这种潜在随机性可能证明在开发Tn1545作为溶纤维真杆菌诱变工具方面是有用的。
