Courvalin P, Carlier C
Mol Gen Genet. 1987 Feb;206(2):259-64. doi: 10.1007/BF00333582.
Tn1545, from Streptococcus pneumoniae BM4200, confers resistance to kanamycin (aphA-3), erythromycin (ermAM) and tetracycline (tetM). The 25.3 kb element is self-transferable to various Gram-positive bacterial genera where it transposes. Tn1545 was cloned in its entirety in the recombination deficient Escherichia coli HB101 where it was unstable. The three resistance genes aphA-3, ermAM and tetM were expressed but were not transferable to other E. coli cells. Tn1545 transposed from the hybrid plasmid to multiple sites of the chromosome of its new host. The element re-transposed, at a frequency of 5 X 10(-9), from the chromosome to various sites of a conjugative plasmid where it could be lost by apparently clean excision. The element transformed and transposed to the chromosome of Bacillus subtilis. The properties of the conjugative shuttle transposon Tn1545 may account for the recent emergence of genes from Gram-positive bacteria in Gram-negative organisms.
来自肺炎链球菌BM4200的Tn1545赋予对卡那霉素（aphA - 3）、红霉素（ermAM）和四环素（tetM）的抗性。这个25.3 kb的元件可自我转移至其发生转座的各种革兰氏阳性细菌属。Tn1545被完整克隆到重组缺陷型大肠杆菌HB101中，在那里它不稳定。三个抗性基因aphA - 3、ermAM和tetM得以表达，但不能转移到其他大肠杆菌细胞。Tn1545从杂交质粒转座到其新宿主染色体的多个位点。该元件以5×10(-9)的频率从染色体重新转座到接合性质粒的各个位点，在那里它可能通过明显的精确切除而丢失。该元件转化并转座到枯草芽孢杆菌的染色体上。接合性穿梭转座子Tn1545的特性可能解释了革兰氏阳性菌的基因最近在革兰氏阴性生物中出现的现象。
