Gasparian G G, Sarkisian N K, Saiadian A V, Grigorian R M
Institute of Zoology, Armenian National Academy of Sciences, Yerevan.
Tsitologiia. 1997;39(7):566-70.
In sparsely seeded (1.10(3) cells/sm2) chick embryo cell cultures no cell proliferation commonly occurs. However, such factors as increasing cell density, a conditioned medium, or addition of ethanol fixed homologous cells to the culture may accelerate the cell growth. The mitogenic action of fixed cells serves as a contact stimulation of cell proliferation (Gasparian, Grigorian, 1989, 1990). Distant and contact cell-to cell interactions, that involve soluble and insoluble cell derived mitogens, are supposed to operate during the log phase of culture growth. The addition of an excess of cells to the previously sparse culture may mimic the cell microenvironment commonly existing in subconfluent cultures. The role of diverse cell-to cell contacts in the cell growth regulation is discussed. The addition of ethanol-fixed cells may improve the cell cloning technique.
在接种稀疏（1.10(3) 个细胞/平方厘米）的鸡胚细胞培养物中，通常不会发生细胞增殖。然而，诸如增加细胞密度、添加条件培养基或向培养物中添加乙醇固定的同源细胞等因素可能会加速细胞生长。固定细胞的促有丝分裂作用可作为细胞增殖的接触刺激（加斯帕里安、格里戈里安，1989年、1990年）。涉及可溶性和不溶性细胞衍生促有丝分裂原的远距离和接触性细胞间相互作用，被认为在培养生长的对数期起作用。向先前稀疏的培养物中添加过量细胞可能会模拟亚汇合培养物中通常存在的细胞微环境。讨论了不同细胞间接触在细胞生长调节中的作用。添加乙醇固定的细胞可能会改进细胞克隆技术。
