Gasparian G, Sarkissian N, Sayadian A, Grigorian R
Laboratory of Genetics, Institute of Zoology of Armenian NAS, 7, Sevak st, Yerevan, 375014, Armenia.
Cell Biol Int. 1998;22(1):51-3. doi: 10.1006/cbir.1997.0204.
There is no cell proliferation in very sparcely plated chick embryo cell cultures. Substituting conditioned medium or adding of ethanol-fixed homologous cells to the cultures accelerates cell colony growth. The mechanism for the mitogenic action of fixed cells is considered to be the contact stimulation of cell proliferation, and addition of extra cells to sparse culture is believed to mimic the cell micro-environment existing in subconfluent cultures. The role of diverse cell-cell contacts in cultured cell growth regulation is discussed. The procedure used (addition of ethanol-fixed cells) may improve normal cell cloning techniques.
在接种细胞极少的鸡胚细胞培养物中没有细胞增殖现象。用条件培养基替代或向培养物中添加乙醇固定的同源细胞可加速细胞集落生长。固定细胞促有丝分裂作用的机制被认为是对细胞增殖的接触刺激，并且向稀疏培养物中添加额外细胞被认为可模拟亚汇合培养物中存在的细胞微环境。讨论了不同细胞间接触在培养细胞生长调节中的作用。所采用的方法（添加乙醇固定的细胞）可能会改进正常的细胞克隆技术。
