Kagami O, Gotoh M, Makino Y, Mohri H, Kamiya R, Ogawa K
Laboratory of Cell Communication, National Institute for Basic Biology, Okazaki 444, Japan.
Gene. 1998 May 12;211(2):383-6. doi: 10.1016/s0378-1119(98)00128-0.
The outer-arm dynein of sea urchin sperm flagella contains six light chains with molecular masses of 23.2, 20.8, 12.3, 11.5, 10.4 and 9. 3kDa. We have cloned a cDNA for the 12.3kDa polypeptide (light chain 3) and found that this protein is highly homologous to mouse Tctex1, a protein encoded by a member of the multigene family in the t complex region that is involved in male sterility and the development of the germ cells. Tctex1 has recently been shown to be homologous to a light chain of cytoplasmic dynein. Therefore, the cytoplasmic dynein light chain has been implicated in the mechanism for the transmission ratio distortion (meiotic drive) that is characteristic of t haplotypes in mice. Our present finding, however, indicates that axonemal light chain 3 must be considered equally important.
海胆精子鞭毛的外臂动力蛋白含有6条轻链，分子量分别为23.2、20.8、12.3、11.5、10.4和9.3 kDa。我们克隆了编码12.3 kDa多肽（轻链3）的cDNA，发现该蛋白与小鼠Tctex1高度同源，Tctex1是t复合区域多基因家族成员编码的一种蛋白，参与雄性不育和生殖细胞发育。最近研究表明Tctex1与胞质动力蛋白的一条轻链同源。因此，胞质动力蛋白轻链与小鼠t单倍型特有的传递比率畸变（减数分裂驱动）机制有关。然而，我们目前的发现表明，轴丝轻链3也同样重要。
