King S M, Dillman J F, Benashski S E, Lye R J, Patel-King R S, Pfister K K
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032-3305, USA.
J Biol Chem. 1996 Dec 13;271(50):32281-7. doi: 10.1074/jbc.271.50.32281.
Mammalian brain cytoplasmic dynein contains three light chains of Mr = 8,000, 14,000, and 22,000 (King, S. M., Barbarese, E., Dillman, J. F., III, Patel-King, R. S., Carson, J. H., and Pfister, K. K. (1996) J. Biol. Chem. 271, 19358-19366). Peptide sequence data (16/16 residues correct) implicate the Mr = 14,000 polypeptide as Tctex-1, a protein encoded within the mouse t-complex. Tctex-1 cosediments with microtubules and is eluted with ATP or salt but not with GTP as expected for a dynein subunit. The ATP-eluted protein precisely cosediments with known cytoplasmic dynein proteins in sucrose density gradients. Tctex-1 also is immunoprecipitated from brain and other tissue homogenates by a monoclonal antibody raised against the 74-kDa cytoplasmic dynein intermediate chain. Quantitative densitometry indicates that Tctex-1 is a stoichiometric component of the dynein complex. As Tctex-1 is a candidate for involvement in the transmission ratio distortion (meiotic drive) of mouse t-haplotypes, these results suggest that cytoplasmic dynein dysfunction may play an important role in non-mendelian chromosome segregation.
哺乳动物脑细胞质动力蛋白包含分子量分别为8000、14000和22000的三条轻链（金，S.M.，巴尔巴雷斯，E.，迪尔曼，J.F.三世，帕特尔 - 金，R.S.，卡森，J.H.，和菲斯特，K.K.（1996年）《生物化学杂志》271，19358 - 19366）。肽序列数据（16个残基全部正确）表明分子量为14000的多肽是Tctex - 1，一种在小鼠t - 复合体中编码的蛋白质。Tctex - 1与微管共沉降，并且如动力蛋白亚基所预期的那样，可被ATP或盐洗脱，但不能被GTP洗脱。在蔗糖密度梯度中，ATP洗脱的蛋白质与已知的细胞质动力蛋白精确共沉降。Tctex - 1也可被针对74 kDa细胞质动力蛋白中间链产生的单克隆抗体从脑和其他组织匀浆中免疫沉淀。定量光密度测定表明Tctex - 1是动力蛋白复合体的化学计量组分。由于Tctex - 1是参与小鼠t - 单倍型传递比扭曲（减数分裂驱动）的候选蛋白，这些结果表明细胞质动力蛋白功能障碍可能在非孟德尔式染色体分离中起重要作用。
