Choi Y, Kim SG
Department of Molecular Biology and Research Center for Cell Differentiation (J.H.A., Y.C., J.S.L.)
Plant Physiol. 1998 Feb 1;116(2):671-9. doi: 10.1104/pp.116.2.671.
A novel extensin gene has been identified in soybean (Glycine max L.) that encodes a hydroxyproline-rich glycoprotein (SbHRGP3) with two different domains. In this study expression of SbHRGP3 was investigated during soybean root development. SbHRGP was expressed in roots of mature plants, as well as seedlings, and showed a distinct pattern of expression during root development. The expression of SbHRGP3 increased gradually during root development of seedlings and reached a maximum while the secondary roots were maturing. The maximum expression level was contributed mainly by the secondary roots rather than by the primary root. Furthermore, expression of SbHRGP3 was preferentially detected in the regions undergoing maturation of the primary and secondary roots. These results imply that the expression of SbHRGP3 is regulated in an organ- and development-specific manner and that in soybean SbHRGP3 expression may be required for root maturation, presumably for the cessation of root elongation. Wounding and sucrose in combination enhanced expression of SbHRGP3 in roots, whereas both wounding and sucrose were required for the expression of SbHRGP3 in leaves.
在大豆（Glycine max L.）中已鉴定出一种新的伸展蛋白基因，该基因编码一种具有两个不同结构域的富含羟脯氨酸的糖蛋白（SbHRGP3）。在本研究中，对SbHRGP3在大豆根系发育过程中的表达进行了研究。SbHRGP在成熟植株和幼苗的根中均有表达，并且在根系发育过程中呈现出独特的表达模式。在幼苗根系发育过程中，SbHRGP3的表达逐渐增加，在侧根成熟时达到最大值。最大表达水平主要由侧根而非主根贡献。此外，在主根和侧根的成熟区域优先检测到SbHRGP3的表达。这些结果表明，SbHRGP3的表达以器官和发育特异性方式受到调控，并且在大豆中，SbHRGP3的表达可能是根系成熟所必需的，大概是为了停止根的伸长。创伤和蔗糖共同作用增强了根中SbHRGP3的表达，而创伤和蔗糖对于叶片中SbHRGP3的表达都是必需的。
