Chaabouni A, Dellacherie E
J Chromatogr. 1979 Apr 1;171:135-43. doi: 10.1016/s0021-9673(01)95293-8.
The partition of the delta 5 leads to 4 3-oxosteroid isomerase of Pseudomonas testosteroni in aqueous two-phase systems containing both the macroligand, polyoxyethylene glycol-bound estradiol, and charged (cationic or anionic) dextrans has been studied. If the enzyme is well retained in the upper phase by an adequate amount of macroligand, it is possible to improve the removal of the contaminating proteins by extracting them into a lower phase containing positively or negatively charged dextran. Some mult-step extraction experiments were carried out to purify isomerase, by washing the upper phase successively with cationic and anionic dextran-rich phases.
在同时含有大环配体（聚乙二醇结合雌二醇）和带电（阳离子或阴离子）葡聚糖的双水相体系中，研究了睾丸酮假单胞菌的δ5-4 3-氧代类固醇异构酶的分配情况。如果通过适量的大环配体将酶很好地保留在上相中，那么通过将污染蛋白提取到含有带正电或带负电葡聚糖的下相中，就有可能提高对污染蛋白的去除效果。通过用上相依次与富含阳离子和阴离子葡聚糖的相进行洗涤，开展了一些多步提取实验来纯化异构酶。
