Susa N, Ueno S, Furukawa Y
Department of Veterinary Public Health, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
J Vet Med Sci. 1998 Jan;60(1):71-6. doi: 10.1292/jvms.60.71.
Pretreatment of primary cultures of rat hepatocytes with sodium diethyldithiocarbamate (DDTC) for 15 min prior to exposure to K2Cr2O7 resulted in a marked decrease in dichromate-induced cytotoxicity, as evaluated by the leakage of lactate dehydrogenase, and in lipid peroxidation, as monitored by malondialdehyde formation. In addition, pretreatment with DDTC attenuated the suppression of the level of vitamin E attributed to K2Cr2O7. However, DDTC pretreatment had no effect on the cellular levels of glutathione or vitamin C or on the activity of the glutathione reductase, glutathione peroxidase, superoxide dismutase or alkaline phosphatase suppressed by dichromate. Under the same experimental conditions, cellular uptake or distribution of chromium was not affected by DDTC. These results indicate that the protective effect of DDTC on chromium (VI)-induced cytotoxicity as well as lipid peroxidation may be associated with the level of nonenzymatic antioxidants such as vitamin E.
在暴露于重铬酸钾（K2Cr2O7）之前，用二乙基二硫代氨基甲酸钠（DDTC）对大鼠肝细胞原代培养物进行15分钟的预处理，结果显示，通过乳酸脱氢酶泄漏评估的重铬酸盐诱导的细胞毒性以及通过丙二醛形成监测的脂质过氧化作用均显著降低。此外，DDTC预处理减弱了重铬酸钾对维生素E水平的抑制作用。然而，DDTC预处理对谷胱甘肽或维生素C的细胞水平以及重铬酸盐抑制的谷胱甘肽还原酶、谷胱甘肽过氧化物酶、超氧化物歧化酶或碱性磷酸酶的活性没有影响。在相同的实验条件下，DDTC不影响细胞对铬的摄取或分布。这些结果表明，DDTC对六价铬诱导的细胞毒性以及脂质过氧化的保护作用可能与维生素E等非酶抗氧化剂的水平有关。
