来自淋巴细胞性白血病细胞系的禽白血病病毒快速诱导淋巴细胞性白血病和腹水。

Rapid induction of lymphoid leukosis and ascites by avian leukosis virus from a lymphoid leukosis cell line.

作者信息

Hihara H, Maeda M, Nakamura K, Ishino S, Tsukamoto K, Yuasa N, Shirai J

机构信息

National Institute of Animal Health, Tsukuba, Ibaraki, Japan.

出版信息

J Vet Med Sci. 1998 Jan;60(1):77-85. doi: 10.1292/jvms.60.77.

DOI:10.1292/jvms.60.77

PMID:9492364

Abstract

To examine whether a lymphoid leukosis (LL) cell line releases an LL-specific avian leukosis virus (ALV) or not, two viral materials, culture fluid and a concentrated viral material from an LL-cell line, were inoculated into a total of 74 day-old chicks of line 15I in 5 experiments. Spectrum of diseases induced, their incidence and incubation periods to onset were examined. Fifteen chicks were inoculated with the culture fluid and 9 (60%) developed ascites [59-119 days post inoculation (dpi); geometric mean (GM) of dpi, GM: 89.6)], but LL was not induced in any chicks inoculated. Fifty-nine chicks were inoculated with the concentrated viral material and LL was recognized in 13 (22.0%) (27-74 dpi; GM: 48.4), ascites with LL in 11 (18.6%) (34-75 dpi; GM: 41.3), ascites alone in 21 (35.6%) (32-83 dpi; GM: 48.2), erythroblastosis in 2 (3.4%) (70-102 dpi; GM: 84.5), and other diseases in 12 (20.3%) (43-102 dpi; GM: 61.8). LL lesions were frequently observed in the liver, spleen, kidneys, bursa of Fabricius (bursa), bone marrow and gonads. Mild lymphocytic foci in some visceral organs and perivascular cuffing in the central nervous system were observed mainly in several chicks diagnosed as having complication of ascites with LL or other diseases. In addition to these lesions, atrophy of bursa and thymuses was recognized in them. No antibodies against Marek's disease virus (MDV) and reticuloendotheliosis virus were detected in 36 sera taken from the chicks inoculated with the concentrated viral material. Serotype 2 MDV was isolated from the buffy coat of some inoculated chicks. These results suggest that the properties of ALV inoculated and immunosuppression caused by inoculation with high doses of ALV are involved in rapid induction of LL and expression of pathogenicity of serotype 2 MDV released from the LL cell line and included in the viral inoculum. This is the first report describing the rapid induction of LL and ascites in chicks.

摘要

为了检测一种淋巴细胞性白血病（LL）细胞系是否释放LL特异性禽白血病病毒（ALV），在5次实验中，将两种病毒材料，即来自LL细胞系的培养液和浓缩病毒材料，接种到总共74只1日龄的15I系雏鸡中。检查所诱发疾病的种类、发病率和发病潜伏期。15只雏鸡接种了培养液，其中9只（60%）出现腹水[接种后59 - 119天（dpi）；dpi的几何平均值（GM），GM：89.6]，但接种的雏鸡均未诱发LL。59只雏鸡接种了浓缩病毒材料，其中13只（22.0%）出现LL（27 - 74 dpi；GM：48.4），11只（18.6%）出现伴有LL的腹水（34 - 75 dpi；GM：41.3），21只（35.6%）仅出现腹水（32 - 83 dpi；GM：48.2），2只（3.4%）出现成红细胞增多症（70 - 102 dpi；GM：84.5），12只（20.3%）出现其他疾病（43 - 102 dpi；GM：61.8）。LL病变常见于肝脏、脾脏、肾脏、法氏囊、骨髓和性腺。一些内脏器官中的轻度淋巴细胞灶以及中枢神经系统中的血管周围套袖现象主要见于几只被诊断为伴有LL或其他疾病腹水并发症的雏鸡。除了这些病变外，还发现它们的法氏囊和胸腺萎缩。从接种浓缩病毒材料的雏鸡采集的36份血清中未检测到抗马立克氏病病毒（MDV）和网状内皮组织增殖病病毒的抗体。从一些接种雏鸡的血沉棕黄层中分离出2型MDV。这些结果表明，接种的ALV的特性以及高剂量ALV接种引起的免疫抑制与LL的快速诱发以及从LL细胞系释放并包含在病毒接种物中的2型MDV的致病性表达有关。这是第一份描述雏鸡快速诱发LL和腹水的报告。