Meier H, Henkel E, Dankert H
J Clin Chem Clin Biochem. 1979 Nov;17(11):709-16.
Alpha-amylase has been measured in 57 patients' sera with normal to highly elevated amylase activities. The results obtained with four enzymatic-kinetic test-kits have been compared with those from five conventional marketed procedures. The results are evaluated by regression analysis. The specificity of the methods was analysed with amylase samples from human pancreas, human saliva and pig pancreas. The susceptibility of the kinetic procedures to interferences is smaller. The problems of conversion of activity units and reference ranges are discussed. The UltraZyme and the Monamyl neu-methods have been found superior to the other procedures because of their good practicability, precision and broad linear range; their specificity for diagnostic purposes in pancreatic diseases is less favourable compared with that of conventional procedures.
已对57例血清淀粉酶活性正常至高度升高的患者血清进行了α淀粉酶检测。将四种酶动力学检测试剂盒所得结果与五种传统市售检测方法的结果进行了比较。通过回归分析对结果进行评估。用人胰腺、人唾液和猪胰腺的淀粉酶样本分析了这些方法的特异性。动力学检测方法受干扰的敏感性较小。讨论了活性单位换算和参考范围的问题。已发现UltraZyme和Monamyl neu方法因其良好的实用性、精密度和较宽的线性范围而优于其他方法;与传统方法相比,它们在胰腺疾病诊断中的特异性不太理想。