Glatz Elisabeth, Persson Martin, Rutberg Blanka
Department of Microbiology, Lund University, Sölvegatan 12, S-223 62 Lund, Sweden.
Microbiology (Reading). 1998 Feb;144 ( Pt 2):449-456. doi: 10.1099/00221287-144-2-449.
The Bacillus subtilis glpD gene encodes glycerol-3-phosphate (G3P) dehydrogenase. Expression of glpD is mainly controlled by termination/antitermination of transcription at an inverted repeat in the glpD leader. Antitermination is mediated by the antiterminator protein GlpP in the presence of G3P. In this paper, interaction between GlpP and glpD leader mRNA in vivo and in vitro is reported. In vivo, the antiterminating effect of GlpP can be titrated in a strain carrying the glpD leader on a plasmid. GlpP has been purified and gel shift experiments have shown that it binds to glpD leader mRNA in vitro. GlpP is not similar to other known antiterminator proteins, but database searches have revealed an Escherichia coli ORF which has a high degree of similarity to GlpP.
枯草芽孢杆菌的glpD基因编码甘油-3-磷酸（G3P）脱氢酶。glpD的表达主要通过在glpD前导序列中的一个反向重复序列处转录的终止/抗终止来控制。在G3P存在的情况下，抗终止由抗终止蛋白GlpP介导。本文报道了GlpP与glpD前导mRNA在体内和体外的相互作用。在体内，GlpP的抗终止作用可以在携带质粒上glpD前导序列的菌株中进行滴定。GlpP已被纯化，凝胶迁移实验表明它在体外与glpD前导mRNA结合。GlpP与其他已知的抗终止蛋白不同，但数据库搜索显示大肠杆菌的一个开放阅读框与GlpP具有高度相似性。
