Glatz E, Farewell A, Rutberg B
Department of Microbiology, Lund University, Sweden.
FEMS Microbiol Lett. 1998 May 1;162(1):93-6. doi: 10.1111/j.1574-6968.1998.tb12983.x.
Expression of the Bacillus subtilis glpD gene which encodes glycerol-3-phosphate (G3P) dehydrogenase is regulated by the GlpP protein which, in the presence of G3P, causes antitermination of transcription of glpD. The glpD gene leader fused to lacZ was integrated into the chromosome of Escherichia coli deleted for the lac operon and carrying the B. subtilis glpP gene on a plasmid. beta-Galactosidase activity of this strain was increased by the addition of G3P. When G3P and glucose, glucose-6-phosphate or fructose-6-phosphate were added, beta-galactosidase activity was reduced showing that GlpP mediates catabolite repression of transcription from the glpD leader in the absence of any other B. subtilis protein.
编码甘油 - 3 - 磷酸（G3P）脱氢酶的枯草芽孢杆菌glpD基因的表达受GlpP蛋白调控，在G3P存在的情况下，GlpP蛋白会导致glpD转录的抗终止。与lacZ融合的glpD基因前导序列被整合到缺失lac操纵子并在质粒上携带枯草芽孢杆菌glpP基因的大肠杆菌染色体中。添加G3P可增加该菌株的β - 半乳糖苷酶活性。当添加G3P和葡萄糖、6 - 磷酸葡萄糖或6 - 磷酸果糖时，β - 半乳糖苷酶活性降低，这表明在没有任何其他枯草芽孢杆菌蛋白的情况下，GlpP介导了对glpD前导序列转录的分解代谢物阻遏。
