Holmberg C, Rutberg B
Department of Microbiology, University of Lund, Sweden.
Mol Microbiol. 1991 Dec;5(12):2891-900. doi: 10.1111/j.1365-2958.1991.tb01849.x.
The Bacillus subtilis glpD gene encodes glycerol-3-phosphate (G3P) dehydrogenase. A sigma A type promoter and the transcriptional startpoint for glpD were identified. Between the transcriptional startpoint and glpD there is an inverted repeat followed by a run of T residues. The inverted repeat prevents expression of a reporter gene, xylE, when positioned between this gene and a constitutive promoter. Expression of xylE, like expression of glpD, is induced by G3P and repressed by glucose. Induction also requires the product of the glpP gene. Our results suggest that glpD expression is controlled by antitermination of transcription. The inverted repeat appears to be a target for induction by G3P and GlpP. We speculate that glucose repression is mediated via an inhibitory effect on synthesis or activity of GlpP.
枯草芽孢杆菌的glpD基因编码甘油-3-磷酸（G3P）脱氢酶。已鉴定出一个σA类型启动子和glpD的转录起始点。在转录起始点和glpD之间有一个反向重复序列，其后跟着一串T残基。当该反向重复序列位于报告基因xylE和组成型启动子之间时，会阻止报告基因的表达。xylE的表达，如同glpD的表达一样，受G3P诱导并受葡萄糖抑制。诱导还需要glpP基因的产物。我们的结果表明，glpD的表达受转录抗终止控制。反向重复序列似乎是G3P和GlpP诱导的靶点。我们推测葡萄糖抑制是通过对GlpP合成或活性的抑制作用介导的。
