离体仓鼠肝细胞对l-[3-³H]乳酸的代谢
The metabolism of l-[3-3h]lactate by isolated hamster liver cells.
作者信息
Rognstad R, Wals P
出版信息
Biochim Biophys Acta. 1976 Jun 23;437(1):16-21. doi: 10.1016/0304-4165(76)90343-3.
The rate of tritium removal from L-[3-3H]lactate by hamster liver cells is faster than the analytical rate of lactate utilization, or the rate of 14C disappearance from 6-[U-14C, 3-3H]Llactate decrease. However, addition of low concentrations (0.1 to 1.0 mM) of L-cycloserine, a glutamate pyruvate transaminase inhibitor, nearly equalizes the rates of isotope utilization from L-[3-3H]lactate and L-[U-14C]lactate. The results suggest a very limited rate of recycling of phosphoenolpyruvate back to pyruvate during gluconeogenesis from lactate in fasted hamster liver cells.
仓鼠肝细胞从L-[3-³H]乳酸中去除³H的速率比乳酸利用的分析速率快，或者说比6-[U-¹⁴C, 3-³H]L-乳酸减少时¹⁴C消失的速率快。然而，添加低浓度（0.1至1.0 mM）的L-环丝氨酸（一种谷氨酸丙酮酸转氨酶抑制剂），几乎使L-[3-³H]乳酸和L-[U-¹⁴C]乳酸的同位素利用速率相等。结果表明，在禁食的仓鼠肝细胞中，从乳酸进行糖异生过程中，磷酸烯醇式丙酮酸再循环回丙酮酸的速率非常有限。
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