Ingle R, Jennings T A, Goodman M L, Pilch B Z, Bergman S, Ross J S
Department of Pathology, Albany Medical College, New York 12208, USA.
Am J Clin Pathol. 1998 Mar;109(3):309-14. doi: 10.1093/ajcp/109.3.309.
Increased expression of the cell adhesion molecule, CD44 standard form (CD44s), has been associated with papillary epithelial tumors, and decreased expression has been linked to tumor invasion and metastasis. Sinonasal inverted papillomas (SIPs) are the most common papillary tumors of the sinonasal tract. This study tests whether the development of squamous cell carcinoma (SCC) in situ and invasive SCC in SIP is associated with altered expression of CD44s. Seventy-six specimens of SIP from 68 patients, 2 specimens of SIP with focal SCC in situ, and 10 specimens of invasive SCC arising in SIP were studied. Automated immunohistochemistry was performed for CD44s expression on paraffin-embedded tissue sections using mouse antihuman CD44 antibody. All 76 SIPs (100%) expressed CD44 (strong membranous staining, 83%; moderate staining, 12%; weak staining, 5%). Two (100%) of 2 SIPs with SCC in situ maintained strong expression in benign and severely dysplastic foci. Six (60%) of 10 SIPs with SCC showed complete loss of CD44s expression, while 4 (40%) of 10 cases of SIP with SCC showed weak expression. Two SIPs with SCC (20%) featured weak diffuse staining of the SCC component, and 2 SIPs with SCC (20%) featured weak focal staining of the SCC component. The non-SCC SIP components of the 10 SIPs with SCC uniformly featured intact membranous CD44 staining. As in other papillary epithelial neoplasms, the typical benign SIP features diffuse membranous CD44s expression. In cases of SIP developing an invasive SCC, CD44s expression in the SCC component is frequently lost.
细胞黏附分子CD44标准型（CD44s）表达增加与乳头状上皮肿瘤相关，而表达降低与肿瘤侵袭和转移有关。鼻窦内翻性乳头状瘤（SIP）是鼻窦最常见的乳头状肿瘤。本研究旨在检测SIP中原位鳞状细胞癌（SCC）和浸润性SCC的发生是否与CD44s表达改变有关。对68例患者的76份SIP标本、2份伴有局灶原位SCC的SIP标本以及10份起源于SIP的浸润性SCC标本进行了研究。使用小鼠抗人CD44抗体对石蜡包埋组织切片进行CD44s表达的自动免疫组织化学检测。所有76份SIP（100%）均表达CD44（强膜染色，83%；中度染色，12%；弱染色，5%）。2份伴有原位SCC的SIP中有2份（100%）在良性和重度发育异常灶中保持强表达。10份伴有SCC的SIP中有6份（60%）显示CD44s表达完全缺失，而10例伴有SCC的SIP中有4份（40%）显示弱表达。2份伴有SCC的SIP（20%）的SCC成分表现为弱弥漫性染色，2份伴有SCC的SIP（20%）的SCC成分表现为弱局灶性染色。10份伴有SCC的SIP的非SCC SIP成分均表现为完整的膜性CD44染色。与其他乳头状上皮肿瘤一样，典型的良性SIP具有弥漫性膜性CD44s表达。在发生浸润性SCC的SIP病例中，SCC成分中的CD44s表达经常缺失。
