Takahashi Y, Bucana C D, Cleary K R, Ellis L M
Department of Surgery, Cancer Research Institute, Kanazawa University, Japan.
Int J Cancer. 1998 Feb 20;79(1):34-8. doi: 10.1002/(sici)1097-0215(19980220)79:1<34::aid-ijc7>3.0.co;2-x.
We previously demonstrated an association between vascular endothelial growth factor (VEGF), vessel counts and metastasis in human colon cancer specimens. Mutant p53 has been implicated in the regulation of angiogenesis. Immuno-histochemical detection of p53 protein has been associated with p53 gene mutations. We sought to determine a correlation between p53 protein detection (i.e., mutant p53), VEGF expression and vessel counts in human colon cancer. Surgical specimens from 93 patients with colon cancer were stained immuno-histochemically for p53, VEGF and factor VIII. Vessel counts were greater in metastatic tumors than in nonmetastatic tumors and adenomas, and greater in nonmetastatic tumors than in adenomas. Vessel counts were highest in tumors with the highest VEGF expression. Vessel counts and VEGF expression were greater in p53-positive tumors than in p53-negative tumors. p53 expression correlated with both VEGF expression and vessel count. The association of p53 expression with VEGF and vessel count suggests that the poor prognosis associated with p53 mutations may be due, in part, to the role of mutant p53 in promoting angiogenesis.
我们先前已证实在人类结肠癌标本中，血管内皮生长因子（VEGF）、血管计数与转移之间存在关联。突变型p53与血管生成的调控有关。p53蛋白的免疫组织化学检测与p53基因突变相关。我们试图确定人类结肠癌中p53蛋白检测（即突变型p53）、VEGF表达与血管计数之间的相关性。对93例结肠癌患者的手术标本进行p53、VEGF和因子VIII的免疫组织化学染色。转移瘤中的血管计数高于非转移瘤和腺瘤，非转移瘤中的血管计数高于腺瘤。VEGF表达最高的肿瘤中血管计数最高。p53阳性肿瘤中的血管计数和VEGF表达高于p53阴性肿瘤。p53表达与VEGF表达和血管计数均相关。p53表达与VEGF和血管计数的关联表明，与p53突变相关的预后不良可能部分归因于突变型p53在促进血管生成中的作用。
