zu Putlitz J, Roberts E A, Wieland S, Kono Y, Blum H E
Department of Internal Medicine II, University Hospital Freiburg, Germany.
Virus Res. 1997 Dec;52(2):177-82. doi: 10.1016/s0168-1702(97)00115-9.
Transient transfection and in vitro infection experiments were performed to characterize replication and antigen synthesis of the hepatitis B virus (HBV) in human hepatocyte lines HH29 and HHY41, derived from normal liver tissue. These liver cell lines are capable of supporting HBV replication and gene expression at levels similar to the human hepatoma cell line HuH-7. Strikingly, a very tight adhesion of HBV to the outer cell membrane of HH29 and HHY41 was observed under conditions that removed HBV to undetectable levels from HuH-7 hepatoma cells. However, no productive HBV infection could be established in these cells as determined by the absence of viral transcripts and de novo antigen synthesis. In conclusion, the human hepatocyte cell lines HH29 and HHY41 may be useful to study important aspects of late steps in the replication of HBV, but appear to lack certain cellular components that play a pivotal role during early steps of the viral life cycle.
进行了瞬时转染和体外感染实验，以表征源自正常肝组织的人肝细胞系HH29和HHY41中乙型肝炎病毒（HBV）的复制和抗原合成。这些肝细胞系能够支持HBV复制和基因表达，其水平与人类肝癌细胞系HuH-7相似。令人惊讶的是，在将HBV从HuH-7肝癌细胞中去除至检测不到水平的条件下，观察到HBV与HH29和HHY41的外细胞膜紧密粘附。然而，由于没有病毒转录本和从头抗原合成，在这些细胞中无法建立有生产性的HBV感染。总之，人肝细胞系HH29和HHY41可能有助于研究HBV复制后期步骤的重要方面，但似乎缺乏在病毒生命周期早期步骤中起关键作用的某些细胞成分。
