Tanner A, Maiden M F, Macuch P J, Murray L L, Kent R L
Forsyth Dental Center, Boston, Massachusetts 02115, USA.
J Clin Periodontol. 1998 Feb;25(2):85-98. doi: 10.1111/j.1600-051x.1998.tb02414.x.
This study compared the subgingival microbiota in periodontal health, gingivitis and initial periodontitis using predominant culture and a DNA probe, checkerboard hybridization method. 56 healthy adult subjects with minimal periodontal attachment loss were clinically monitored at 3-month intervals for 12 months. More sites demonstrated small increments of attachment loss than attachment gain over the monitoring period. Sites, from 17 subjects, showing > or = 1.5 mm periodontal attachment loss during monitoring were sampled as active lesions for microbial analysis. Twelve subjects demonstrated interproximal lesions, and 5 subjects had attachment loss at buccal sites (recession). Cultural studies identified Bacteroides forsythus, Campylobacter rectus, and Selenomonas noxia as the predominant species associated with active interproximal lesions (9 subjects), whereas Actinomyces naeslundii, and Streptococcus oralis, were the dominant species colonizing buccal active sites. A. naeslundii, Campylobacter gracilis, and B. forsythus (at lower levels than active sites) were the dominant species cultured from gingivitis (10 subjects). Health-associated species (10 subjects) included Streptococcus oralis, A. naeslundii, and Actinomyces gerencseriae. DNA probe data identified higher mean levels of B. forsythus and C. rectus with active (7 subjects) compared to inactive periodontitis sites. Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were detected infrequently. Cluster analysis of the cultural microbiota grouped 8/9 active interproximal lesions in one subcluster characterized by a mostly gram-negative microbiota, including B. forsythus and C. rectus. The data suggest that B. forsythus C. rectus and S. noxia were major species characterizing sites converting from periodontal health to disease. The differences in location and microbiota of interproximal and buccal active sites suggested that different mechanisms may be involved in increased attachment loss.
本研究采用优势培养法及DNA探针棋盘杂交法，比较了牙周健康、牙龈炎及早期牙周炎患者的龈下微生物群。对56名牙周附着丧失极小的健康成年受试者进行临床监测，为期12个月，每隔3个月检查一次。在监测期内，显示附着丧失增加的部位多于附着增加的部位。选取17名受试者中在监测期间牙周附着丧失≥1.5 mm的部位作为活跃病变部位进行微生物分析。12名受试者出现邻面病变，5名受试者颊侧部位有附着丧失（牙龈退缩）。培养研究确定，福赛坦氏菌、直肠弯曲菌和具核梭杆菌是与活跃邻面病变相关的主要菌种（9名受试者），而内氏放线菌和口腔链球菌是颊侧活跃部位的优势定植菌。内氏放线菌、纤细弯曲菌和福赛坦氏菌（数量低于活跃部位）是从牙龈炎患者（10名受试者）中培养出的优势菌种。与健康相关的菌种（10名受试者）包括口腔链球菌、内氏放线菌和杰氏放线菌。DNA探针数据显示，与非活动性牙周炎部位相比，活跃性牙周炎部位（7名受试者）的福赛坦氏菌和直肠弯曲菌平均水平更高。牙龈卟啉单胞菌和伴放线放线杆菌很少被检测到。对培养出的微生物群进行聚类分析，结果显示将9个活跃邻面病变中的8个归为一个亚群，其特征为主要是革兰氏阴性微生物群，包括福赛坦氏菌和直肠弯曲菌。数据表明，福赛坦氏菌、直肠弯曲菌和具核梭杆菌是表征从牙周健康转变为疾病部位的主要菌种。邻面和颊侧活跃部位在位置和微生物群方面的差异表明，附着丧失增加可能涉及不同机制。
