Hindy A M, Abdelhaleem E S, Aly R H
Oral Surgery Department, Faculty of Oral and Dental medicine, Cairo University.
Egypt Dent J. 1995 Jul;41(3):1217-26.
The present study was conducted on 105 subjects, 70 dentists working or studying at the Faculty of Oral and Dental Medicine, Cairo University, and 35 non medical non dental subjects. There were 47 males and 48 females, their ages ranged from 20-50 years. Detailed history and the required information were collected from each subject and were recorded in a specially a prepared questionnaire study cards. 10 C.C. of venous blood were obtained from each candidate using Venoject, sera were separated from clotted blood by centrifuge; their sera were tested for Hepatitis B surface antigen (HBsAg), Antibody to hepatitis B surface antigen (anti HBs), Antibody to hepatitis B core antigen (anti HBc) and Antibody to hepatitis C virus antigen (anti HCV) using ELISA (enzyme linked immuno sorbent assay) techniques according to Abbott Laboratories (West Germany). The ALT level (Alanine aminotransferase) was determined only for HBsAg and anti HCV positive cases using commercial Biomérieux kits (France). After statistical analysis of the results, the exposure rate of HBV among dentists was found to be 27.1% with a carrier rate of 7.1% compared to 31.4% with a carrier rate of 17.1% in the control group. The exposure rate of HCV infection among dentists was 1.4% compared to 17.1% in the control group. The exposure rate of HBV and HCV infections were 2.9% compared to 5.7% in the control group; these results and other important conclusions were adequately discussed.
本研究共纳入105名受试者,其中70名是在开罗大学口腔与牙科学院工作或学习的牙医,35名是非医学非牙科专业人员。受试者中男性47名,女性48名,年龄在20至50岁之间。从每位受试者处收集详细病史及所需信息,并记录在专门准备的问卷研究卡片上。使用真空采血管从每位受试者采集10毫升静脉血,通过离心机从凝血中分离血清;根据德国雅培实验室的方法,采用酶联免疫吸附测定(ELISA)技术检测血清中的乙肝表面抗原(HBsAg)、乙肝表面抗体(抗-HBs)、乙肝核心抗体(抗-HBc)和丙肝病毒抗体(抗-HCV)。仅对HBsAg和抗-HCV阳性病例使用法国生物梅里埃公司的商用试剂盒测定丙氨酸氨基转移酶(ALT)水平。对结果进行统计分析后发现,牙医中乙肝病毒(HBV)暴露率为27.1%,携带率为7.1%,而对照组的暴露率为31.4%,携带率为17.1%。牙医中丙肝病毒(HCV)感染暴露率为1.4%,而对照组为17.1%。HBV和HCV感染的暴露率为2.9%,而对照组为5.7%;对这些结果及其他重要结论进行了充分讨论。